Veterinary School. Federal University of Minas Gerais (UFMG), Antônio Carlos Avenue, 6627, 31.270-901, Belo Horizonte, MG, Brazil.
Veterinary School. Federal University of Minas Gerais (UFMG), Antônio Carlos Avenue, 6627, 31.270-901, Belo Horizonte, MG, Brazil.
Anaerobe. 2019 Feb;55:24-28. doi: 10.1016/j.anaerobe.2018.10.002. Epub 2018 Oct 4.
One of the main challenges associated with Clostridioides difficile infection (CDI) in humans and domestic animals is the lack of an effective preventive strategy. One strategy with promising results is the oral administration of non-toxigenic strains of C. difficile (NTCD). Recently, Z31, a NTCD strain isolated from a healthy dog, showed promising results to prevent CDI in hamsters. Thus, the present study aimed to evaluate the capacity of Z31 to prevent CDI in piglets using an experimental model. Twenty neonatal piglets were randomly distributed in three groups: G1 - 10 spores of Z31 followed by 10 spores of a toxigenic C. difficile strain (n = 7), G2 (positive control) - 10 spores of a toxigenic C. difficile strain (n = 7), and G3 (negative control) - no biological inoculum (n = 6). All animals were kept in individual insulators and observed for 60 h. Data regarding clinical signs, macro and microscopic lesions, toxigenic culture of C. difficile, and detection of A/B toxins in the feces were evaluated. All evaluated parameters were significantly lower in animals that received Z31 compared to the positive control. Thus, oral administration of Z31 was able to prevent CDI in piglets in an experimental model.
艰难梭菌感染（CDI）在人和家畜中面临的主要挑战之一是缺乏有效的预防策略。一种具有前景的策略是口服非产毒艰难梭菌（NTCD）菌株。最近，从健康犬中分离出的 NTCD 菌株 Z31 显示出在仓鼠中预防 CDI 的有希望的结果。因此，本研究旨在使用实验模型评估 Z31 预防仔猪 CDI 的能力。将 20 头新生仔猪随机分为三组：G1 - 10 个 Z31 孢子，随后是 10 个产毒艰难梭菌菌株（n=7），G2（阳性对照）- 10 个产毒艰难梭菌菌株（n=7），G3（阴性对照）- 无生物接种物（n=6）。所有动物均单独隔离并观察 60 小时。评估了临床症状、宏观和微观病变、艰难梭菌产毒培养以及粪便中 A/B 毒素的检测数据。与阳性对照组相比，接受 Z31 治疗的动物的所有评估参数均显著降低。因此，口服 Z31 能够在实验模型中预防仔猪 CDI。
