Department of Orthopaedics, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi, China.
J Cell Physiol. 2019 Aug;234(8):13832-13842. doi: 10.1002/jcp.28063. Epub 2019 Jan 13.
Wearing titanium particle-induced osteoclastogenesis, accompanied by peri-implant osteolysis, is the main cause of long-term failure of hip prosthesis. Currently, medications used for the prevention and treatment of peri-implant osteolysis show serious side effects. Therefore, development for more effective new drugs with less side effects is extremely urgent. Vaccarin is a natural flavonoid extracted from Vaccaria segetalis, with various biological functions, including antioxidantory, anti-inflammatory, and promotion of angiogenesis. However, the putative role of vaccarin in the inhibition of titanium particle-induced osteolysis has not been reported. In this study, it was indicated that vaccarin could effectively inhibit RANKL-induced osteoclastogenesis, fusion of F-actin rings, bone resorption, and expression of osteoclast marker genes in a dose-dependent manner in vitro. Moreover, vaccarin could also inhibit RANKL-induced osteoclastogenesis via the inhibition of NF-κB and MAPK (p38, ERK, and JNK) signaling pathways, and inhibit the transcription of downstream transcription factors, such as c-Fos and NFATc1. Consistent with in vitro results, this in vivo study showed that vaccarin exhibited an inhibitory effect on titanium particle-induced osteolysis by antiosteoclastogenesis. In conclusion, vaccarin could be a promising agent for preventing and treating peri-implant osteolysis.
钛颗粒诱导破骨细胞形成，伴随种植体周围骨溶解，是髋关节假体长期失效的主要原因。目前，用于预防和治疗种植体周围骨溶解的药物显示出严重的副作用。因此，开发更有效、副作用更小的新药是极其紧迫的。舞茸素是从金顶侧耳中提取的一种天然黄酮类化合物，具有多种生物学功能，包括抗氧化、抗炎和促进血管生成。然而，舞茸素在抑制钛颗粒诱导的骨溶解中的作用尚未报道。在这项研究中，表明舞茸素可以在体外有效抑制 RANKL 诱导的破骨细胞分化、F-肌动蛋白环融合、骨吸收以及破骨细胞标记基因的表达，呈剂量依赖性。此外，舞茸素还可以通过抑制 NF-κB 和 MAPK（p38、ERK 和 JNK）信号通路来抑制 RANKL 诱导的破骨细胞分化，并抑制下游转录因子如 c-Fos 和 NFATc1 的转录。与体外结果一致，这项体内研究表明，舞茸素通过抗破骨细胞形成对钛颗粒诱导的骨溶解具有抑制作用。总之，舞茸素可能是预防和治疗种植体周围骨溶解的一种有前途的药物。
