Animal Reproduction, Gynaecology & Obstetrics, ICAR-National Dairy Research Institute, Karnal, India.
Andrologia. 2019 May;51(4):e13233. doi: 10.1111/and.13233. Epub 2019 Jan 13.
The cryopreservation of sperm is a well established technique that plays an essential role in dissemination of elite germplasm of livestock. Despite having numerous advantages, the cryopreservation induces certain stresses on sperm including structural and functional damages leading to impaired sperm quality and fertility, which might be associated with production of reactive oxygen species (ROS). In addition, the ROS upon reacting with sperm lipids, DNA and proteins may lead to a cascade of sperm damages. The sperm membrane contains a rich amount of polyunsaturated fatty acids, which increases their susceptibility to oxidative stress induced damages, leading to formation of secondary products. These secondary products result in oxidation of sperm proteins via carbonylation. The carbonylation could lead to disturbances in specific proteins that are involved in capacitation. The present review deals with sperm protein carbonylation.
精子的冷冻保存是一项成熟的技术，在传播家畜的优良种质方面发挥着重要作用。尽管有许多优点，但冷冻保存会对精子造成一定的压力，包括结构和功能的损伤，导致精子质量和生育能力下降，这可能与活性氧（ROS）的产生有关。此外，ROS 与精子脂质、DNA 和蛋白质反应会导致一连串的精子损伤。精子膜含有丰富的多不饱和脂肪酸，这增加了它们对氧化应激诱导损伤的敏感性，导致次级产物的形成。这些次级产物通过羰基化导致精子蛋白的氧化。羰基化可能导致参与顶体反应的特定蛋白质发生紊乱。本文综述了精子蛋白羰基化。
