Udoekong Ekaette Chris, Ramirez-Lopez Camilo Jose, Silva Okano Denise, Barros Edvaldo, Pereira Vidigal Pedro Marcus, Ribeiro Iara Magalhães, Rodrigues Carvalho Renner Philipe, Machado-Neves Mariana, Guimarães José Domingos, Facioni Guimarães Simone Eliza
Laboratory of Animal Biotechnology, Department of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.
Laboratory of Animal Reproduction, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.
Int J Mol Sci. 2025 Mar 10;26(6):2457. doi: 10.3390/ijms26062457.
Sexual rest (SR) in bulls leads to the accumulation of senescent spermatozoa in the extragonadal reserves, potentially affecting semen quality and reproductive efficiency. Therefore, this study aimed to investigate the impact of SR on the seminal plasma proteome and oxidative status of Nellore bulls. Six adult bulls were subjected to 195 days of SR and sequential semen collections using the electroejaculation method. The ejaculates were analyzed to assess sperm quality. Seminal plasma from the first and last ejaculates was evaluated for oxidative status and proteomic profile using LC-MS. The results revealed significant improvements in sperm motility, vigor, and antioxidant enzyme activity (superoxide dismutase and catalase) in the last ejaculate compared to the first. Conversely, higher levels of oxidative markers, such as malondialdehyde and carbonyl proteins, were observed in the first ejaculate. Proteomic analysis identified 156 proteins, with 28 differentially abundant between ejaculates. The first ejaculate showed a higher abundance of proteins linked to acrosomal exocytosis and energy metabolism, while proteins associated with sperm motility and immune modulation were elevated in the last ejaculate. These findings suggest that SR induces oxidative stress and proteomic alterations in seminal plasma, negatively affecting sperm quality, emphasizing the need for strategic reproductive management in bulls.
公牛的性休息（SR）会导致性腺外储备中衰老精子的积累，这可能会影响精液质量和繁殖效率。因此，本研究旨在调查性休息对Nellore公牛精浆蛋白质组和氧化状态的影响。六头成年公牛经历了195天的性休息，并采用电射精法进行连续采精。对射出的精液进行分析以评估精子质量。使用液相色谱-质谱联用技术（LC-MS）对首次和末次射精的精浆进行氧化状态和蛋白质组分析。结果显示，与首次射精相比，末次射精的精子活力、活力和抗氧化酶活性（超氧化物歧化酶和过氧化氢酶）有显著改善。相反，在首次射精中观察到较高水平的氧化标志物，如丙二醛和羰基蛋白。蛋白质组分析鉴定出156种蛋白质，其中有28种在两次射精之间差异丰富。首次射精显示与顶体胞吐作用和能量代谢相关的蛋白质丰度较高，而与精子活力和免疫调节相关的蛋白质在末次射精中升高。这些发现表明，性休息会诱导精浆中的氧化应激和蛋白质组改变，对精子质量产生负面影响，强调了公牛进行战略繁殖管理的必要性。
