NaCl 对沸石咪唑酯骨架-8 包埋酶的影响。
The effects of NaCl on enzyme encapsulation by zeolitic imidazolate frameworks-8.
机构信息
Nanjing University of Science and Technology, Department of Bioengineering, Xiaolingwei 200, 210094, Nanjing, China.
Nanjing University of Science and Technology, Department of Bioengineering, Xiaolingwei 200, 210094, Nanjing, China.
出版信息
Enzyme Microb Technol. 2019 Mar;122:1-6. doi: 10.1016/j.enzmictec.2018.12.003. Epub 2018 Dec 3.
Metal-organic framework (MOF) has generated a lot of research interests for it can be employed as immobilization material for enzyme. There are many small molecules in enzyme solution during the extraction process, such as NaCl. It is important to study the effects of the small molecules on MOFs. Here we report a facile and efficient method to encapsulate (R)-1-phenylethanol dehydrogenase ((R)-PEDH) into zeolitic imidazolate framework-8 (ZIF-8). In this work, the effects of NaCl on shape of ZIF-8 and enzyme encapsulation have been investigated. The scanning electron microscope (SEM) results showed that 0.1 M NaCl affect the morphology of ZIF-8 while the crystal structure was not changed analyzed by X-ray diffraction (XRD), and 0.1 M NaCl consisted in the encapsulation system of (R)-PEDH@ZIF-8 enhanced the activity up to 2.5 folds than no NaCl consisted during prepared (R)-PEDH@ZIF-8. The (R)-PEDH@ZIF-8 (prepared with 0.1 M NaCl) enhanced the storage stability and resist ability against trypsin, and the (R)-PEDH@ZIF-8 has been successfully reused.
金属-有机骨架（MOF）作为酶的固定化材料引起了广泛的研究兴趣。在提取过程中，酶溶液中有许多小分子，如 NaCl。研究小分子对 MOF 的影响非常重要。在这里，我们报道了一种简便高效的方法，将（R）-1-苯乙醇脱氢酶（（R）-PEDH）包封到沸石咪唑酯骨架-8（ZIF-8）中。在这项工作中，研究了 NaCl 对 ZIF-8 形状和酶包封的影响。扫描电子显微镜（SEM）结果表明，0.1 M NaCl 影响 ZIF-8 的形态，而 X 射线衍射（XRD）分析表明其晶体结构没有变化，并且 0.1 M NaCl 存在于（R）-PEDH@ZIF-8 的包封体系中，使酶的活性提高了 2.5 倍，而在制备（R）-PEDH@ZIF-8 时没有 NaCl。（R）-PEDH@ZIF-8（用 0.1 M NaCl 制备）提高了储存稳定性和对胰蛋白酶的抗性，并且（R）-PEDH@ZIF-8 已成功重复使用。
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