Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou, 510070, Guangdong, China.
Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou, 510070, Guangdong, China.
Anal Biochem. 2019 Mar 15;569:10-15. doi: 10.1016/j.ab.2019.01.001. Epub 2019 Jan 9.
A novel method for non-target screening of toxicants in poisonous honey was established in this study. Poisonous honey and nontoxic honey were contrastive detected using liquid chromatography quadrupole-time-of-flight mass spectrometry and analyzed by Mass Profiler Professional Software. 4 poisonous alkaloids were screened out and confirmed by comparison with reference compounds. In order to investigate the source of these poisonous alkaloids, 6 poisonous alkaloids, ubiquitous in Gelsemium elegan, from honey, honeybees, pollen in honeycomb and different organs of Gelsemium elegan, were quantified by liquid chromatography triple-quadrupole tandem mass spectrometry. The results showed that alkaloids composition characteristics in honey, honeybees, and pollen were similar to those in the flower and bud of Gelsemium elegan and significant different from those in leave, stem and root. This result demonstrated that poisonous alkaloids in honey were come from the gathering honey process. This strategy provided an efficient and rapid method for non-target screening of toxicants in food.
本研究建立了一种用于检测毒蜜中毒素的非靶向筛选新方法。采用液相色谱-四极杆飞行时间质谱法对毒蜜和无毒蜜进行对比检测，并利用 Mass Profiler Professional 软件进行分析。通过与对照品比较，筛选出 4 种有毒生物碱并得到确认。为了探究这些有毒生物碱的来源，采用液相色谱三重四极杆串联质谱法对来源于毒蜜、蜜蜂、蜂巢花粉及钩吻不同器官中的 6 种普遍存在于钩吻中的生物碱进行了定量分析。结果表明，蜂蜜、蜜蜂和花粉中的生物碱组成特征与钩吻花和花蕾中的相似，与钩吻叶、茎和根中的显著不同。这一结果表明，蜂蜜中的有毒生物碱来自于采蜜过程。该策略为食品中毒素的非靶向筛选提供了一种高效快速的方法。
