Guedes Israel Barbosa, Araújo Simone Aparecida de Almeida, de Souza Gisele Oliveira, de Souza Silva Sheila Oliveira, Taniwaki Sueli Akemi, Cortez Adriana, Brandão Paulo Eduardo, Heinemann Marcos Bryan
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
Instituto Federal de Educação Ciência e Tecnologia do Estado do Pará, Cametá, PA, Brazil.
Acta Trop. 2019 Mar;191:212-216. doi: 10.1016/j.actatropica.2019.01.011. Epub 2019 Jan 11.
The present study aimed to detect the most prevalent serogroups and circulating Leptospira species in cows from Brazilian Amazon. Samples of blood serum, urine and kidney of 208 animals were collected at a municipal slaughterhouse in the Baixo Tocantins region of Pará State, Northern Brazil. The tests used were microscopic agglutination test (MAT), bacteriological isolation, polymerase chain reaction (PCR) and DNA sequencing. The frequency of MAT-reactive cows was 46.6% (97/208) with titers ranging from 100 to 3200, being Sejroe serogroup the most prevalent. There was no Leptospira isolation, but the DNA of bacterium was detected in 5.8% (12/208) of the kidney and in 14.9% (31/208) of the urine samples. DNA sequencing was performed directly from PCR products of 30 samples (3 kidneys and 27 urines), with identification of four different species: L. borgpetersenii with 56.7% (17/30), followed by L. kirschneri with 13.3% (4/30), L. interrogans with 6.7% (2/30), L. santarosai with 3.3% (1/30), and 20.0% (6/30) of samples were identified only at the genus level. These results reveal a diversity and peculiarity for bovine leptospirosis in the Amazon region, mainly due to the low frequency of L. santarosai and more surprising, the presence of L. kirschneri, differently of what is observed in other regions of Brazil.
本研究旨在检测巴西亚马逊地区奶牛中最常见的血清群和流行的钩端螺旋体种类。在巴西北部帕拉州下托坎廷斯地区的一个市级屠宰场，采集了208头动物的血清、尿液和肾脏样本。所使用的检测方法有显微镜凝集试验（MAT）、细菌分离、聚合酶链反应（PCR）和DNA测序。MAT反应阳性的奶牛频率为46.6%（97/208），滴度范围为100至3200，其中Sejroe血清群最为常见。未分离到钩端螺旋体，但在5.8%（12/208）的肾脏和14.9%（31/208）的尿液样本中检测到了该细菌的DNA。对30个样本（3个肾脏和27个尿液）的PCR产物直接进行DNA测序，鉴定出四种不同的种类：博氏钩端螺旋体占56.7%（17/30），其次是克氏钩端螺旋体占13.3%（4/30），问号钩端螺旋体占6.7%（2/30），桑塔罗斯钩端螺旋体占3.3%（1/30），20.0%（6/30）的样本仅在属水平上被鉴定出来。这些结果揭示了亚马逊地区牛钩端螺旋体病的多样性和特殊性，主要是由于桑塔罗斯钩端螺旋体的低频率，更令人惊讶的是克氏钩端螺旋体的存在，这与巴西其他地区的情况不同。
