Medeiros Luciana Dos Santos, Braga Domingos Susan Christina, Azevedo Maria Isabel Nogueira Di, Peruquetti Rui Carlos, de Albuquerque Narianne Ferreira, D'Andrea Paulo Sérgio, Botelho André Luis de Moura, Crisóstomo Charle Ferreira, Vieira Anahi Souto, Martins Gabriel, Teixeira Bernardo Rodrigues, Carvalho-Costa Filipe Anibal, Lilenbaum Walter
Laboratório de Microbiologia e Imunologia Veterinária, Universidade Federal do Acre, Rio Branco, Brazil.
Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Niterói, Brazil.
Front Vet Sci. 2020 Dec 2;7:569004. doi: 10.3389/fvets.2020.569004. eCollection 2020.
is a bacteria that causes leptospirosis and is transmitted through water, soil, or mud that is contaminated by the urine of infected animals. Although it is mainly associated with the urban environment, Leptospires also circulate in rural and wild environments. This study aimed to investigate the role of small mammals in leptospirosis epidemiology in the western Amazon, Brazil. In total, 103 animals from 23 species belonging to the orders Didelphimorphia and Rodentia were captured. Blood, kidney, and urine samples were collected and Microscopic Agglutination Test (MAT), L32 PCR, Y sequencing, and culturing were conducted. MAT was reactive on 1/15 sera, and no bacterial isolate was obtained. PCR yielded 44.7% positive samples from 16 species. Twenty samples were genetically characterized and identified as ( = 12), ( = 4), and ( = 4). No statistical association was found between the prevalence of infection by spp. in small mammals within carrier/hosts species, orders, study area, and forest strata. Our results indicate a high prevalence of pathogenic spp. in several rodent and marsupial species and report the first evidence of spp. carrier/hosts in the Brazilian Western Amazon.
是一种可引发钩端螺旋体病的细菌，通过受感染动物尿液污染的水、土壤或泥浆传播。尽管它主要与城市环境相关，但钩端螺旋体也在农村和野外环境中传播。本研究旨在调查小型哺乳动物在巴西亚马逊西部钩端螺旋体病流行病学中的作用。总共捕获了来自23个物种、属于袋鼬目和啮齿目的103只动物。采集了血液、肾脏和尿液样本，并进行了显微镜凝集试验（MAT）、L32聚合酶链反应（PCR）、Y测序和培养。MAT在1/15份血清上呈阳性反应，未获得细菌分离株。PCR从16个物种中获得了44.7%的阳性样本。对20个样本进行了基因特征分析，鉴定为（=12）种、（=4）种和（=4）种。在携带/宿主物种、目、研究区域和森林地层内的小型哺乳动物中，未发现感染 spp. 的患病率之间存在统计学关联。我们的结果表明，几种啮齿动物和有袋动物物种中致病性 spp. 的患病率很高，并报告了巴西亚马逊西部 spp. 携带/宿主的首个证据。
