Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
Médico Veterinário, Auditor Fiscal Agropecuário, Agência de Defesa e Inspeção Agropecuária do Estado do Amapá, Brazil.
Vet Med Sci. 2020 Aug;6(3):433-440. doi: 10.1002/vms3.271. Epub 2020 Apr 21.
Although Brazil has one of the largest buffalo populations in the Americas, buffalo leptospirosis is still poorly explored when compared to that in bovines; thus, the aim of this research was to carry out a large serological study for leptospirosis in this species in the Brazilian Amazon. For this, we collected 1,405 serum samples from buffaloes raised in the Amazon delta region, which is considered a major area of buffalo production in Brazil. The test used was a microscopic agglutination test (MAT) adopting 34 Leptospira antigens, some of which have never been tested for buffaloes in Brazil, including autochthonous strains; in total, 20 serogroups were evaluated. From the total of 1,405 serum samples, 894 (63.6%) reacted in the MAT to at least one of the 20 serogroups, and 511 (36.4%) did not react. The serogroups Sejroe, Autumnalis and Pomona were the most prevalent, with titres ranging from 100 to 12,800, and the autochthonous strains used were not significant in relation to the reference serovars. Leptospirosis in buffaloes seems to have a serological profile similar to leptospirosis in cattle, mainly due to the prevalence of the Sejroe serogroup; however, the results of this study suggested that in the Brazilian Amazon, Leptospira strains that are serologically distinct from the autochthonous strains isolated in the southeastern region of Brazil may be circulating in these animals. Other serovars could also be inserted into the panel of antigens used in MAT for serological studies on buffaloes.
尽管巴西拥有美洲最大的水牛种群之一，但与牛相比，水牛钩端螺旋体病的研究仍不够深入；因此，本研究旨在对巴西亚马逊地区的这种物种进行大规模的钩端螺旋体病血清学研究。为此，我们从位于亚马逊三角洲地区的水牛中收集了 1405 份血清样本，该地区被认为是巴西主要的水牛生产区。所用的检测方法是显微镜凝集试验（MAT），采用 34 种钩端螺旋体抗原，其中一些在巴西从未用于水牛检测，包括地方分离株；总共评估了 20 个血清群。在总共 1405 份血清样本中，有 894 份（63.6%）在 MAT 中对至少 20 个血清群中的一个产生反应，511 份（36.4%）未产生反应。血清群 Sejroe、Autumnalis 和 Pomona 最为流行，滴度范围为 100 至 12800，所用的地方分离株与参考血清型相比并不重要。水牛钩端螺旋体病的血清学特征似乎与牛钩端螺旋体病相似，主要是由于 Sejroe 血清群的流行；然而，本研究的结果表明，在巴西亚马逊地区，与巴西东南部分离的地方分离株在血清学上不同的钩端螺旋体菌株可能在这些动物中传播。其他血清型也可以被插入用于水牛血清学研究的 MAT 中使用的抗原组中。
