Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 5;212:167-172. doi: 10.1016/j.saa.2019.01.001. Epub 2019 Jan 3.
Hematin is an oxidized form of heme, and the abnormal levels of hematin in the human body can lead to various inflammatory lesions. Hence, there is still a need to establish a rapid, sensitive and efficient method for hematin detection. Herein, the green fluorescent carbon quantum dots (CQDs) are synthesized by using L-cysteine and hydrogen peroxide as precursors. The synthesized CQDs exhibit some fascinating characters including excellent water solubility, high fluorescence quantum yield, and good stability in a broad pH range of 7.0-11.0 and high ionic strength solution. Excitingly, the fluorescence of CQDs can be rapidly and selectively quenched by hematin via the inner filter effect. Moreover, the detection of hematin by the CQDs fluorescent probe shows a good linearity in the concentration range of 0.5-30 μM with a minimum detection limit of 0.1 μM. Finally, the proposed approach is successfully applied to detect hematin in human blood samples.
血红素是血红素的氧化形式，人体中血红素水平异常会导致各种炎症病变。因此，仍然需要建立一种快速、灵敏和高效的血红素检测方法。本文以 L-半胱氨酸和过氧化氢为前体合成了绿色荧光碳量子点（CQDs）。所合成的 CQDs 具有一些迷人的特性，包括优异的水溶性、高荧光量子产率以及在 7.0-11.0 的宽 pH 范围和高离子强度溶液中的良好稳定性。令人兴奋的是，血红素可以通过内滤效应快速和选择性地猝灭 CQDs 的荧光。此外，血红素的检测通过 CQDs 荧光探针在 0.5-30 μM 的浓度范围内显示出良好的线性关系，最小检测限为 0.1 μM。最后，该方法成功应用于检测人血样中的血红素。
