State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
Chemosphere. 2019 Apr;221:132-140. doi: 10.1016/j.chemosphere.2019.01.047. Epub 2019 Jan 7.
The increasing finding of pathogens and antibiotic resistance genes (ARGs) in drinking water has become one of the most challenging global health threats worldwide. However, conventional disinfection strategies in drinking water treatment plants (DWTPs) require further optimization in combating the antibiotic resistome. Here, we show that antimicrobial resins with quaternary ammonium salts (AMRs-QAS) exhibit great potentials in diminishing specific potential pathogens that relatively resist chlorine or UV disinfection in DWTPs, and comprehensive analyses using microscopy and fluorescence techniques revealed that the antimicrobial capacity of AMRs-QAS mainly proceed via the bacterial adsorption and cell membrane dissociation. Moreover, a total of 15 among 30 selected ARGs, as well as 4 selected potential pathogens including Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Staphylococcus aureus were all detected in the source water. Coupling the AMRs-QAS with 0.2 mg/L chlorine resulted in higher removal efficiencies than chlorination (2 mg/L) or UV disinfection (400 mJ cm) for all the detected pathogens and ARGs in drinking water and significantly decreased the relative abundances of Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, as well as all the detected ARGs (p < 0.05). Co-occurrences of pathogens and ARGs were revealed by a correlation network and possibly accounts for the ARGs removal. This coupled disinfection strategy overcomes the limitations of individual disinfection methods, i.e. the enrichment of specific pathogens and ARGs among bacterial populations, and provides an alternative for minimizing health risks induced by the antibiotic resistome in DWTPs.
饮用水中病原体和抗生素耐药基因（ARGs）的发现不断增加，已成为全球范围内最具挑战性的健康威胁之一。然而，饮用水处理厂（DWTP）中的常规消毒策略在对抗抗生素耐药组方面需要进一步优化。在这里，我们表明，带有季铵盐（AMRs-QAS）的抗菌树脂在减少 DWTP 中相对抵抗氯或 UV 消毒的特定潜在病原体方面具有巨大潜力，使用显微镜和荧光技术的综合分析表明，AMRs-QAS 的抗菌能力主要通过细菌吸附和细胞膜解离来实现。此外，在源水中共检测到 30 个选定 ARGs 中的 15 个，以及包括铜绿假单胞菌、枯草芽孢杆菌、大肠杆菌和金黄色葡萄球菌在内的 4 种选定的潜在病原体。与 0.2mg/L 氯结合使用的 AMRs-QAS 对饮用水中所有检测到的病原体和 ARGs 的去除效率均高于氯化（2mg/L）或 UV 消毒（400mJ/cm），并显著降低了铜绿假单胞菌、枯草芽孢杆菌、大肠杆菌的相对丰度，以及所有检测到的 ARGs（p<0.05）。病原体和 ARGs 的共存通过相关网络揭示，并可能解释了 ARGs 的去除。这种组合消毒策略克服了单一消毒方法的局限性，即在细菌种群中特定病原体和 ARGs 的富集，并为 DWTP 中最小化抗生素耐药组引起的健康风险提供了一种替代方法。
