Section of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
Clinical Institute of Medical and Laboratory Diagnostics, Medical University of Graz, Austria.
J Clin Virol. 2019 Feb;111:29-32. doi: 10.1016/j.jcv.2019.01.003. Epub 2019 Jan 7.
For infection control measures, rapid accurate diagnostics on admission of patients with suspected seasonal influenza is crucial.
Prospective comparison of three rapid molecular tests for detection of influenza A/B RNA.
Outpatients presenting at the Medical emergency department of Graz University Hospital with influenza-like illness and a requirement for hospitalization (n = 312) were studied. Nasopharyngeal swabs were collected with the 3 mL-version of the UTM™ Viral Transport Medium (Copan). Specimens were tested for influenza A and B RNA using the Alere™ i Influenza A & B (Abbott), the cobas Influenza A/B (Roche), and the Xpert Xpress Flu/RSV (Cepheid) tests. Results were compared to those obtained from the same specimen by the Influenza A/B R-GENE (bioMerieux) test based on real-time PCR as reference method.
Overall sensitivities of the Abbott, Roche, and Cepheid tests were 90.5%, 96.0%, and 97.0%, overall specificities 99.4%, 97.6%, and 98.2% respectively. With the Abbott and the Cepheid tests, all specimens gave valid results, while the Roche test showed invalid results in 37 (12.1%) specimens. Total time to result for the Abbott, Roche, and Cepheid tests was 18 min, 22 min, and 32 min respectively.
The Abbott test lacked sensitivity, the Roche test was impaired by a high number of invalid results. Overall, despite the longest total time to result, the Cepheid test showed the best performance to detect influenza virus RNA in symptomatic patients presenting at an emergency unit in this study.
对于感染控制措施，对疑似季节性流感患者入院时进行快速准确的诊断至关重要。
前瞻性比较三种用于检测流感 A/B RNA 的快速分子检测方法。
对格拉茨大学医院急诊部出现流感样症状并需要住院的 312 例门诊患者进行研究。使用 3mL 版 UTM™ 病毒转运培养基（Copan）采集鼻咽拭子。使用 Alere™ i 流感 A & B（雅培）、cobas Influenza A/B（罗氏）和 Xpert Xpress Flu/RSV（Cepheid）检测试剂盒检测流感 A 和 B RNA。将结果与基于实时 PCR 的相同标本的Influenza A/B R-GENE（bioMerieux）检测结果进行比较，后者为参考方法。
雅培、罗氏和 Cepheid 检测的总体敏感性分别为 90.5%、96.0%和 97.0%，总体特异性分别为 99.4%、97.6%和 98.2%。雅培和 Cepheid 检测的所有标本均得出有效结果，而罗氏检测的 37 份（12.1%）标本显示无效结果。雅培、罗氏和 Cepheid 检测的总结果时间分别为 18 分钟、22 分钟和 32 分钟。
雅培检测的敏感性不足，罗氏检测因大量无效结果而受到影响。总的来说，尽管 Cepheid 检测的总结果时间最长，但在本研究中，该检测在检测出现症状并到急诊就诊的患者的流感病毒 RNA 方面表现出最佳性能。
