Feizi Sepehr, Delfazayebaher Siamak, Masoudi Ali, Babamohamadi Zahra, Javadi Mohammad Ali
Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Ophthalmology, Urmia University of Medical Sciences, Urmia, Iran.
Cornea. 2019 Apr;38(4):403-408. doi: 10.1097/ICO.0000000000001855.
To compare donor lenticule thickness reported by the eye bank with lenticule thickness measured at 6 months after Descemet stripping automated endothelial keratoplasty (DSAEK).
This prospective, interventional case series enrolled 30 eyes from 30 patients who underwent DSAEK using precut eye bank tissue. Preoperative thickness measurements of the donor lenticules were taken centrally and at pericentral and peripheral graft areas using an anterior segment optical coherence tomography system. The same measurements were repeated 6 months postoperatively. The preoperative and postoperative values were compared, and the relationships between these measurements were investigated using Pearson correlation coefficients.
The mean donor age was 47.7 ± 10.7 years. The mean preoperative endothelial cell density was 3017 ± 361 cells/mm, which decreased to 1645 ± 482 cells/mm postoperatively (P < 0.001). The graft thickness of the precut tissue, as reported by the eye bank, was 136.8 ± 24.9 μm at the center, 149.4 ± 27.3 μm at the pericentral area, and 198.4 ± 32.8 μm at the periphery. The corresponding postoperative values were 119.2 ± 29.8 μm (P = 0.004), 124.5 ± 34.6 μm (P = 0.002), and 156.3 ± 45.4 μm (P < 0.001), respectively. The graft thickness measured by the eye bank was significantly associated with that measured in vivo at 6 months at the central (r = 0.63, P = 0.001) and pericentral (r = 0.50, P = 0.009) graft areas. However, the correlation between preoperative and postoperative peripheral graft thickness was insignificant (P = 0.14).
Compared with preoperative values, central, pericentral, and peripheral graft thicknesses were reduced by 10.4%, 14.2%, and 18.4%, respectively, 6 months after DSAEK. Postoperative graft thinning was greater at the periphery compared with the central and pericentral areas.
比较眼库报告的供体角膜瓣厚度与Descemet膜剥除自动内皮角膜移植术（DSAEK）后6个月测量的角膜瓣厚度。
本前瞻性干预性病例系列纳入了30例患者的30只眼，这些患者使用预先切割的眼库组织接受了DSAEK。使用眼前节光学相干断层扫描系统在中央、中央周边和周边移植区域对供体角膜瓣进行术前厚度测量。术后6个月重复相同测量。比较术前和术后值，并使用Pearson相关系数研究这些测量值之间的关系。
供体平均年龄为47.7±10.7岁。术前平均内皮细胞密度为3017±361个细胞/mm²，术后降至1645±482个细胞/mm²（P<0.001）。眼库报告的预先切割组织的移植厚度在中央为136.8±24.9μm，在中央周边区域为149.4±27.3μm，在周边为198.4±32.8μm。相应的术后值分别为119.2±29.8μm（P = 0.004）、124.5±34.6μm（P = 0.002）和156.3±45.4μm（P<0.001）。眼库测量的移植厚度与术后6个月体内测量的中央（r = 0.63，P = 0.001）和中央周边（r = 0.50，P = 0.009）移植区域的厚度显著相关。然而，术前和术后周边移植厚度之间的相关性不显著（P = 0.14）。
与术前值相比，DSAEK术后6个月，中央、中央周边和周边移植厚度分别降低了10.4%、14.2%和18.4%。与中央和中央周边区域相比，术后周边移植变薄更明显。
