Kanavi Mozhgan Rezaei, Chamani Tahereh, Kheiri Bahareh, Javadi Mohammad Ali
Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Central Eye Bank of Iran, Tehran, Iran.
Cell Tissue Bank. 2018 Dec;19(4):623-628. doi: 10.1007/s10561-018-9712-3. Epub 2018 Jul 14.
This study was conducted to analyze the preoperative thickness profile and endothelial rating of ultrathin Descemet's stripping automated endothelial keratoplasty (UT-DSAEK) tissues prepared with a single versus double microkeratome pass from donated whole eyes and corresponding eye bank postoperative results. Microkeratome-assisted UT-DSAEK tissues were prepared from freshly donated whole eyes with single-pass (SP) and double-pass (DP) technique in the Central Eye Bank of Iran. Preoperative thickness profiles and endothelial cell densities of UT-DSAEK tissues were obtained from optical coherence tomography and specular microscopy, respectively, and compared between groups. Corneal perforation rates during the eye bank preparation and postoperative reports of transplanted UT-DSAEK tissues were also compared. Over a 15-month period, 342 UT-DSAEK tissues were prepared: 248 via SP and 94 with DP technique. Mean donor corneal central thickness was 610 ± 58 µm with SP and 790 ± 100 µm with DP technique. Mean central thickness of UT-DSAEK tissues was not statistically different between the groups (84.8 ± 11.0 µm with SP and 85.1 ± 10.5 µm with DP technique, P = 0.857). Mean increase of UT-DSAEK thickness from central to pericentral and peripheral cornea was not significantly different with both techniques. Mean differences between thicknesses of 2 pericentral locations and between those of 2 peripheral locations were not statistically different in the study groups. Corneal perforation of 1.6 and 1.1% occurred in SP and DP groups, respectively. Failed graft was reported 6 months postoperatively in 4 (1.6%) cases with SP and in 1 (1.1%) case with DP technique. Preoperative thickness profiles of UT-DSAEK tissues prepared from donated whole eyes via SP technique were not significantly different from those prepared with DP, showing a symmetric increase of thickness towards peripheral locations.
本研究旨在分析使用单刀和双刀角膜刀从捐献全眼球制备的超薄Descemet膜剥除自动内皮角膜移植术（UT-DSAEK）组织的术前厚度分布和内皮评级，以及相应的眼库术后结果。在伊朗中央眼库，采用单刀（SP）和双刀（DP）技术从新鲜捐献的全眼球制备角膜刀辅助的UT-DSAEK组织。分别通过光学相干断层扫描和镜面显微镜获得UT-DSAEK组织的术前厚度分布和内皮细胞密度，并在组间进行比较。还比较了眼库制备过程中的角膜穿孔率以及移植UT-DSAEK组织的术后报告。在15个月的时间里，共制备了342个UT-DSAEK组织：248个采用SP技术，94个采用DP技术。SP技术组供体角膜中央平均厚度为610±58μm，DP技术组为790±100μm。两组UT-DSAEK组织的中央平均厚度无统计学差异（SP技术组为84.8±11.0μm，DP技术组为85.1±10.5μm，P = 0.857）。两种技术下UT-DSAEK厚度从中央到周边角膜的平均增加量无显著差异。研究组中两个周边位置的厚度之间以及两个周边位置的厚度之间的平均差异无统计学意义。SP组和DP组的角膜穿孔率分别为1.6%和1.1%。术后6个月，SP技术组有4例（1.6%）报告移植失败，DP技术组有1例（1.1%）报告移植失败。通过SP技术从捐献全眼球制备的UT-DSAEK组织的术前厚度分布与DP技术制备的无显著差异，显示厚度向周边位置呈对称增加。
