Australian Herbicide Resistance Initiative, School of Agriculture and Environment , University of Western Australia , Perth WA 6009 , Australia.
J Agric Food Chem. 2019 Feb 6;67(5):1353-1359. doi: 10.1021/acs.jafc.8b05974. Epub 2019 Jan 25.
There have been many studies on target-site resistance (TSR) to PSII-inhibiting herbicides, but only a few on the non-target-site resistance (NTSR). Here, we reported both TSR and NTSR to metribuzin in a wild radish population. Dose-response studies revealed a higher level of resistance to metribuzin in the resistant (R) compared to the susceptible (S) population. Sequencing of the target psbA gene revealed the known Ser-264-Gly mutation in R plants. In addition, a higher level of [C]-metribuzin metabolism and, consequently, a lower level of [C] translocation were also detected in the R plants. These results demonstrated that both psbA gene mutation and enhanced metabolism contribute to metribuzin resistance in this wild radish population. Furthermore, this resistant population showed resistance to ALS-inhibiting herbicides due to multiple ALS gene mutations. This is the first report in wild radish of metabolic herbicide resistance, in addition to the target-site psbA gene mutation.
已经有许多关于 PSII 抑制性除草剂靶标抗性（TSR）的研究，但关于非靶标抗性（NTSR）的研究却很少。在这里，我们报道了一个野萝卜种群对二甲戊灵的靶标和非靶标抗性。剂量反应研究表明，抗性（R）种群对二甲戊灵的抗性水平高于敏感（S）种群。对靶标 psbA 基因的测序揭示了 R 植株中已知的 Ser-264-Gly 突变。此外，还检测到 R 植株中 [C]-二甲戊灵代谢水平更高，因此 [C]转运水平更低。这些结果表明，野萝卜种群对二甲戊灵的抗性既与 psbA 基因突变有关，也与增强的代谢有关。此外，由于多个 ALS 基因突变，该抗性种群对 ALS 抑制性除草剂也表现出抗性。这是除靶标 psbA 基因突变外，野萝卜中首次报道代谢性除草剂抗性。
