College of Food Science and Engineering , Northwest A&F University , Yangling , Shaanxi 712100 , People's Republic of China.
Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture , Yangling , Shaanxi 712100 , China.
J Agric Food Chem. 2019 Jan 30;67(4):1277-1283. doi: 10.1021/acs.jafc.8b06253. Epub 2019 Jan 22.
A fluorescent method for detection of tetracyclines (TCs) in milk was developed by using the NH-MIL-53(Al) nanosensor synthesized via a one-pot hydrothermal method. The nanosensor had a crystalline nanoplates structure with rich groups of -NH and -COOH. The -NH/-COOH of NH-MIL-53(Al) reacted with the -CO-/-OH of TCs to form a complex. The electron of -NH/-COOH from the NH-BDC ligand transferred to the -CO-/-OH of TCs. -NH of the NH-MIL-53(Al) interacted with the -CO-/-OH of TCs by hydrogen bonding. The quenching efficiency of the inner filter effect (IFE) was calculated to contribute 57-89%. The synergistic effect of photoinduced electron transfer (PET) and IFE account for fluorescence quenching. TCs were quantitatively detected in milk samples with recoveries of 85.15-112.13%; the results were in great accordance with high-performance liquid chromatography (HPLC) ( P > 0.05), confirming the NH-MIL-53(Al) nanosensor has potential applicability for the detection of TCs in food matrix.
采用一锅水热法合成了 NH-MIL-53(Al)纳米传感器，建立了一种用于牛奶中环丙沙星（TCs）检测的荧光方法。该纳米传感器具有丰富的 -NH 和 -COOH 基团的结晶纳米片结构。NH-MIL-53(Al)的 -NH/-COOH 与 TCs 的 -CO-/-OH 反应形成配合物。来自 NH-BDC 配体的 -NH/-COOH 中的电子转移到 TCs 的 -CO-/-OH 上。NH-MIL-53(Al)中的 -NH 通过氢键与 TCs 的 -CO-/-OH 相互作用。内滤效应（IFE）的猝灭效率计算值为 57-89%。光诱导电子转移（PET）和 IFE 的协同效应导致荧光猝灭。在牛奶样品中定量检测 TCs 的回收率为 85.15-112.13%；与高效液相色谱（HPLC）的结果非常吻合（P＞0.05），证实 NH-MIL-53(Al)纳米传感器在检测食品基质中的 TCs 方面具有潜在的适用性。
