Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago de Chile, Chile.
Centro Interdisciplinario de Neurociencias de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso, Valparaiso, Chile.
PLoS Genet. 2019 Jan 14;15(1):e1007863. doi: 10.1371/journal.pgen.1007863. eCollection 2019 Jan.
Many neurons are unable to regenerate after damage. The ability to regenerate after an insult depends on life stage, neuronal subtype, intrinsic and extrinsic factors. C. elegans is a powerful model to test the genetic and environmental factors that affect axonal regeneration after damage, since its axons can regenerate after neuronal insult. Here we demonstrate that diapause promotes the complete morphological regeneration of truncated touch receptor neuron (TRN) axons expressing a neurotoxic MEC-4(d) DEG/ENaC channel. Truncated axons of different lengths were repaired during diapause and we observed potent axonal regrowth from somas alone. Complete morphological regeneration depends on DLK-1 but neuronal sprouting and outgrowth is DLK-1 independent. We show that TRN regeneration is fully functional since animals regain their ability to respond to mechanical stimulation. Thus, diapause induced regeneration provides a simple model of complete axonal regeneration which will greatly facilitate the study of environmental and genetic factors affecting the rate at which neurons die.
许多神经元在受损后无法再生。在受到损伤后再生的能力取决于生命阶段、神经元亚型、内在和外在因素。秀丽隐杆线虫是一个强大的模型，可以用来测试影响损伤后轴突再生的遗传和环境因素，因为它的轴突在神经元损伤后可以再生。在这里，我们证明休眠促进表达神经毒性 MEC-4(d) DEG/ENaC 通道的截断触感器神经元 (TRN) 轴突的完全形态再生。在休眠期间，不同长度的截断轴突被修复，我们观察到来自体的强烈的轴突再生。完全形态再生依赖于 DLK-1，但神经元的发芽和生长是不依赖于 DLK-1 的。我们表明，TRN 的再生是完全功能性的，因为动物重新获得了对机械刺激的反应能力。因此，休眠诱导的再生提供了一个完全轴突再生的简单模型，这将极大地促进研究影响神经元死亡速度的环境和遗传因素。
