Black S J, van der Loo W, Loken M R, Herzenberg L A
J Exp Med. 1978 Apr 1;147(4):984-96. doi: 10.1084/jem.147.4.984.
B lymphocytes capable of generating primary IgM and IgG plaque-forming cells (PFC) responses to burro erythrocytes have surface IgD, as do primary IgM PFC. IgG memroy cells arising after one injection of antigen are divided into two groups, one of which expresses surface IgD while the other has no detectable membrane IgD. PFC generated from the IgG memory cells lacking surface IgD show a higher average avidity than those arising from IgD-positive IgG memory cells, indicating that mature IgG memory cells do not have surface IgD. After more than one injection of antigen, few, if any, IgG memory cells have surface IgD. IgG PFC arising in primary or secondary immune response lack membrane-bound IgD. These data provide the outlines for a B-cell maturation pathway in which IgD marks unprimed and early memory B cells and is lost in mature memory cells. Studies presented here were conducted by isolating IgD+ and IgD- cells with the fluorescence-activated cell sorter and functional testing of the isolated populations in adoptive transfer experiments.
能够对驴红细胞产生原发性IgM和IgG空斑形成细胞（PFC）反应的B淋巴细胞具有表面IgD，原发性IgM PFC也如此。一次注射抗原后产生的IgG记忆细胞分为两组，其中一组表达表面IgD，而另一组则检测不到膜IgD。缺乏表面IgD的IgG记忆细胞产生的PFC比IgD阳性IgG记忆细胞产生的PFC平均亲和力更高，这表明成熟的IgG记忆细胞没有表面IgD。多次注射抗原后，即使有，也很少有IgG记忆细胞具有表面IgD。在原发性或继发性免疫反应中产生的IgG PFC缺乏膜结合IgD。这些数据为B细胞成熟途径勾勒出了轮廓，其中IgD标记未致敏和早期记忆B细胞，并在成熟记忆细胞中丢失。这里介绍的研究是通过用荧光激活细胞分选仪分离IgD+和IgD-细胞，并在过继转移实验中对分离出的群体进行功能测试来进行的。
