Department of Food Science and Experimental Nutrition, School of Pharmaceutical Science , University of São Paulo , São Paulo 05508-000, Brazil.
Food Research Center (FoRC) , CEPID-FAPESP (Research Innovation and Dissemination Centers Sao Paulo Research Foundation) , São Paulo 05468-901 , Brazil.
J Agric Food Chem. 2019 Feb 6;67(5):1381-1391. doi: 10.1021/acs.jafc.8b05408. Epub 2019 Jan 25.
We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. "Cara Cara" and cv. "Bahia" on faecal microbiota and metabolome using an integrated meta-omics approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on H NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group.
我们采用整合宏基因组学方法研究了摄入两种不同的脐橙（Citrus sinensis cv. "Cara Cara" 和 cv. "Bahia"）橙汁对粪便微生物群和代谢组的影响。采用随机交叉设计，健康受试者每天饮用 500ml Cara Cara 或 Bahia 橙汁或等热量对照饮料。在干预前（T0）和干预一周后（T7）收集粪便。针对 16S rRNA 进行焦磷酸测序以获得操作分类单元（OTUs），并通过基于 H NMR 光谱的非靶向代谢组学方法分析粪便代谢物。橙汁摄入后观察到的微生物群组成的主要变化是 Mogibacteriaceae、Tissierellaceae、Veillonellaceae、Odoribacteraceae 和 Ruminococcaceae 家族的 Clostridia OTUs 的丰度增加，其成员受 Cara Cara 或 Bahia 橙汁消费的影响不同。与 Bahia 组相比，Cara Cara 组中有 6 种核心代谢物（如肌醇、胆碱、赖氨酸、精氨酸、尿刊酸和甲酸盐）显著增加。
