Volina E G, Arimitsu Y, Kobayashi S, Kiktenko V S, Tkachenko E A
Zh Mikrobiol Epidemiol Immunobiol. 1988 Nov(11):90-4.
The sensitivity of microcapsular leptospiral antigens, produced by Japan Lyophilization Laboratory and intended for use in tests for the detection of antibodies to leptospires in the sera of experimentally immunized laboratory animals, were studied. The comparative study of the microcapsular agglutination (MCA) test and other serological tests, such as the microagglutination (MA) test and the indirect enzyme immunoassay (EIA), was made. The leptospiral antigens under study were found to actively react with serospecific and group-specific antibodies. In infected guinea pigs and rabbits specific antibodies could be detected from days 3-4 in the MCA test and only from days 5-7 in the MA test. The average antibody level determined by titration in the MCA test was 3.3 times higher and in indirect EIA, 4.3 times higher than that determined by titration in the MA test. These data make it possible to recommend the use of microcapsular leptospiral antigens for the early diagnosis of leptospirosis.
对日本冻干实验室生产的微囊钩端螺旋体抗原进行了研究，该抗原用于检测实验免疫的实验动物血清中钩端螺旋体抗体。对微囊凝集（MCA）试验与其他血清学试验，如微量凝集（MA）试验和间接酶免疫测定（EIA）进行了比较研究。所研究的钩端螺旋体抗原被发现能与血清特异性和群特异性抗体发生积极反应。在感染的豚鼠和兔子中，MCA试验在第3 - 4天就能检测到特异性抗体，而MA试验仅在第5 - 7天才能检测到。通过MCA试验滴定测定的平均抗体水平比MA试验滴定测定的高3.3倍，在间接EIA中高4.3倍。这些数据使得推荐使用微囊钩端螺旋体抗原进行钩端螺旋体病的早期诊断成为可能。
