Cirone S M, Riemann H P, Ruppanner R, Behymer D E, Franti C E
J Wildl Dis. 1978 Apr;14(2):193-202. doi: 10.7589/0090-3558-14.2.193.
Sera from 153 wild animals of 18 species were tested for antibodies against 12 serovars of Leptospira by the microscopic agglutination (MA) test. Seventy-five percent of the animals tested were seropositive against one or more of the 12 serovars used. The most commonly found serovars were pomona, autumnalis, pyrogenes, icterohaemorrhagiae, australis, and canicola. Of 62 carnivores representing 7 species, 55 (89%) were seropositive, as were 46 (60%) of 77 rodents from 9 species. Leptospira of the serovar copenhageni serogroup icterohaemorrhagiae were recovered from kidney tissues of a Norway rat (Rattus norvegicus). Of 443 wildlife sera tested by the indirect hemagglutination (IHA) test using cells sensitized with L. illini antigen, 47 (11%), mainly carnivores and deer, gave a heterophile reaction. Of the remaining 396 sera, 164 (41%) were seropositive for leptospirosis by the IHA test. To compare the IHA test with the MA test, 143 serum samples were tested by both methods. There was 84% concordance between the two tests.
通过显微镜凝集（MA）试验，对18个物种的153只野生动物的血清进行了针对12种钩端螺旋体血清型抗体的检测。所检测动物中有75%对所使用的12种血清型中的一种或多种呈血清阳性。最常见的血清型是波摩那型、秋季热型、致热型、出血黄疸型、澳洲型和犬型。在代表7个物种的62只食肉动物中，55只（89%）呈血清阳性，9个物种的77只啮齿动物中有46只（60%）呈血清阳性。从一只褐家鼠（Rattus norvegicus）的肾脏组织中分离出了出血黄疸型血清群哥本哈根血清型钩端螺旋体。在用伊利诺伊钩端螺旋体抗原致敏细胞的间接血凝（IHA）试验中，对443份野生动物血清进行检测，其中47份（11%），主要是食肉动物和鹿，出现了嗜异性反应。在其余396份血清中，164份（41%）通过IHA试验呈钩端螺旋体病血清阳性。为了比较IHA试验和MA试验，用两种方法对143份血清样本进行了检测。两种试验的一致性为84%。
