Andrews P C, Alai M, Cotter R J
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Anal Biochem. 1988 Oct;174(1):23-31. doi: 10.1016/0003-2697(88)90514-3.
Californium-252 plasma desorption mass spectrometry (252Cf PDMS) of a crude, desalted, extract of piscine endocrine pancreas provided mass information for the major biologically active peptide hormones present in this tissue. An extraction procedure compatible with 252Cf PDMS analysis was developed. In extracts of catfish pancreas, strong molecular ions were identified in the positive mode for somatostatin-14 (1638 amu), O-glycosylated somatostatin-22 (2944 amu), glucagon (3512 amu), glucagon-like peptide (3785 amu), insulin (ca. 5550 amu), and other prohormone-derived peptides. Both protonated species and sodium adducts were apparent in the mass spectrum. A number of other molecular ions were observed including somatostatin-26, 1-10 (1014 amu) and the entire portion of prosomatostatin-22 remaining after removal of somatostatin-22 (6465 amu). The data obtained by this method also resulted in the identification of the third major product of proglucagon processing in catfish pancreas, glicentin-related polypeptide. Subtractive Edman degradation analyzed by 252Cf PDMS was also used to confirm a mass assignment.
对鱼内分泌胰腺的粗提、脱盐提取物进行锎 - 252 等离子体解吸质谱分析（252Cf PDMS），可获取该组织中主要生物活性肽激素的质量信息。开发了一种与 252Cf PDMS 分析兼容的提取方法。在鲶鱼胰腺提取物中，在正模式下鉴定出了生长抑素 - 14（1638 原子质量单位）、O - 糖基化生长抑素 - 22（2944 原子质量单位）、胰高血糖素（3512 原子质量单位）、胰高血糖素样肽（3785 原子质量单位）、胰岛素（约 5550 原子质量单位）以及其他前激素衍生肽的强分子离子。质子化物种和钠加合物在质谱中均很明显。还观察到了许多其他分子离子，包括生长抑素 - 26、1 - 10（1014 原子质量单位）以及去除生长抑素 - 22 后剩余的前生长抑素 - 22 的整个部分（6465 原子质量单位）。通过该方法获得的数据还鉴定出了鲶鱼胰腺中胰高血糖素原加工的第三种主要产物，即胰高血糖素相关多肽。通过 252Cf PDMS 分析的减法埃德曼降解也用于确认质量归属。
