Tian Yingjie, Yu Hui, Wang Zhanli
The Second Affiliated Hospital, Baotou Medical College, 30 Hude Mulin Street, Baotou, 014030, China.
BMC Res Notes. 2019 Jan 15;12(1):27. doi: 10.1186/s13104-019-4064-z.
This study investigated the distribution of acquired antibiotic resistance genes in Enterococcus species isolated from clinical patients in Baotou, China.
A total of 73 enterococca lisolates from clinical samples were collected from December 2016 to September 2017. Of the 73 enterococcal isolates, 36 (49.3%), 35 (47.9%), 1 (1.4%), and 1 (1.4%) were identified as E. faecium, E. faecalis, E. gallinarum, and E. raffinosus, respectively. The resistance rates of the enterococci to nitrofurantoin, tetracycline, gentamicin (high-level), ampicillin, ciprofloxacin and erythromycin were 24.7%, 49.3%, 50.7%, 54.8%, 74.0% and 89.0%, respectively. The most prevalent aminoglycoside resistance genes were aac(6')-Ie-aph(2″)-Ia (64.9%) and aph(3')IIIa (64.9%). The most common erythromycin ribosome methylation gene was erm(B) (67.7%), followed by erm(A) (4.6%) and erm(C) (1.5%). The tetracycline resistance gene tetM was found to be present in 100.0% of the tetracycline-resistant strains of enterococci. Thus, E. faecium and E. faecalis were identified as the species of greatest clinical importance associated with hospital-acquired enterococcal infections in Baotou, China. The antimicrobial resistance genes aac(6')-Ie-aph(2″)-Ia, aph(3')IIIa, tetM, and erm(B) were significantly more prevalent among the enterococcal isolates. Therefore, action should be taken to monitor drug resistance and antimicrobial resistance genes to manage multi-drug-resistant enterococcal infections.
本研究调查了从中国包头临床患者中分离出的肠球菌属中获得性抗生素耐药基因的分布情况。
2016年12月至2017年9月共收集了73株临床样本中的肠球菌分离株。在这73株肠球菌分离株中，分别有36株（49.3%）、35株（47.9%）、1株（1.4%）和1株（1.4%）被鉴定为屎肠球菌、粪肠球菌、鹑鸡肠球菌和棉子糖肠球菌。肠球菌对呋喃妥因、四环素、庆大霉素（高水平）、氨苄西林、环丙沙星和红霉素的耐药率分别为24.7%、49.3%、50.7%、54.8%、74.0%和89.0%。最常见的氨基糖苷类耐药基因是aac(6')-Ie-aph(2″)-Ia（64.9%）和aph(3')IIIa（64.9%）。最常见的红霉素核糖体甲基化基因是erm(B)（67.7%），其次是erm(A)（4.6%）和erm(C)（1.5%）。发现四环素耐药基因tetM存在于100.0%的肠球菌四环素耐药菌株中。因此，屎肠球菌和粪肠球菌被确定为与中国包头医院获得性肠球菌感染相关的具有最大临床重要性的菌种。抗菌耐药基因aac(6')-Ie-aph(2″)-Ia、aph(3')IIIa、tetM和erm(B)在肠球菌分离株中显著更普遍。因此，应采取行动监测耐药性和抗菌耐药基因，以管理多重耐药肠球菌感染。
