Department of Infectious Diseases & Immunology, University of Florida, Gainesville, FL, USA.
Division of Gastroenterology, Hepatology & Nutrition, Department of Medicine, University of Florida, Gainesville, FL, USA.
Mucosal Immunol. 2019 Mar;12(2):434-444. doi: 10.1038/s41385-018-0125-1. Epub 2019 Jan 15.
Newborns are highly susceptible to pathogenic infections with significant worldwide morbidity possibly due to an immature immune system. Recently, we reported that Propionibacterium strain, P. UF1, isolated from the gut microbiota of preterm infants, induced the differentiation of bacteria-specific Th17 cells. Here, we demonstrate that P. UF1 significantly increased the number of protective Th17 cells and maintained IL-10 regulatory T cells (Tregs) in newborn mice. In addition, P. UF1 protected mice from intestinal Listeria monocytogenes (L. m) infection. P. UF1 also functionally sustained the gut microbiota and induced critical B vitamin metabolites implicated in the regulation of T cell immunity during L. m intestinal infection. Transcriptomic analysis of P. UF1-induced Th17 cells revealed genes involved in the differentiation and regulation of these cells. These results illustrate the potency of P. UF1 in the enhancement of neonatal host defense against intestinal pathogen infection.
新生儿极易受到病原感染，具有很高的发病率，这可能是由于其免疫系统不成熟。最近，我们报道了从早产儿肠道微生物群中分离出的丙酸杆菌菌株 P. UF1 可诱导细菌特异性 Th17 细胞分化。在这里，我们证明 P. UF1 可显著增加保护性 Th17 细胞的数量，并维持新生小鼠中的 IL-10 调节性 T 细胞（Tregs）。此外，P. UF1 可保护小鼠免受肠道李斯特菌（L. m）感染。P. UF1 还可维持肠道微生物群的功能，并诱导在 L. m 肠道感染期间参与调节 T 细胞免疫的关键 B 族维生素代谢物。对 P. UF1 诱导的 Th17 细胞的转录组分析显示了涉及这些细胞分化和调节的基因。这些结果说明了 P. UF1 在增强新生儿宿主防御肠道病原体感染方面的强大作用。
