Afify Mei, Kamel Refaat R, Elhosary Yasser A, Hegazy Abdelfattah E, Fahim Hoda H, Ezzat Wafaa M
Biochemistry Dept., National Research Centre, Cairo, Egypt.
Surgery Dept., Faculty of Medicine, Ain Shams University, Cairo, Egypt.
J Genet Eng Biotechnol. 2015 Dec;13(2):157-163. doi: 10.1016/j.jgeb.2015.10.002. Epub 2015 Oct 31.
Hepatocellular carcinoma (HCC) is regarded as one of the most common malignancies and among the leading causes of cancer death in the whole world. Apoptosis is a fundamental process controlling cell death and plays a critical role in normal development of multicellular organisms. When abnormalities occur in apoptosis, a variety of diseases are caused, including cancer. The aim of the current study was to determine the serum expression of Cytochrome c and PDCD4 among patients with hepatocellular carcinoma and chronic hepatitis.
A total of 40 serum and tissue samples (17 samples from chronic hepatitis and 23 samples from HCC patients) were collected. Apoptotic markers in serum were carried out using the quantitative sandwich enzyme immunoassay technique.
We found that serum levels of PCDC4 and Cytochrome c were increased in patients with HCC when compared to chronic hepatitis patients. They were also increased in patients with chronic hepatitis when compared to controls ( < 0.05, significant). Analyzing the impact of HCC characters on serum values of PDCD4 and Cytochrome c revealed that the mean values of both PDCD4 and Cytochrome c are significantly higher in cases with single lesion of HCC ( < 0.05, significant). Right lobe location of HCC lesions has the highest mean values of PDCD4 ( < 0.05, significant). As regards grade of differentiation, grade П has higher mean values of Cytochrome c ( < 0.05, significant).
Serum levels of Cytochrome c and PDCD4 are increased in patients with cirrhosis and hepatocellular carcinoma and could be used as diagnostic aid for HCC.
肝细胞癌（HCC）被认为是最常见的恶性肿瘤之一，也是全球癌症死亡的主要原因之一。细胞凋亡是控制细胞死亡的基本过程，在多细胞生物体的正常发育中起着关键作用。当细胞凋亡出现异常时，会引发多种疾病，包括癌症。本研究的目的是确定肝细胞癌患者和慢性肝炎患者血清中细胞色素c和PDCD4的表达情况。
共收集了40份血清和组织样本（17份来自慢性肝炎患者，23份来自肝细胞癌患者）。血清中的凋亡标志物采用定量夹心酶免疫分析技术进行检测。
我们发现，与慢性肝炎患者相比，肝细胞癌患者血清中PCDC4和细胞色素c的水平升高。与对照组相比，慢性肝炎患者血清中这两种物质的水平也升高（P<0.05，具有显著性）。分析肝细胞癌特征对PDCD4和细胞色素c血清值的影响发现，肝细胞癌单发病灶患者中PDCD4和细胞色素c的平均值显著更高（P<0.05，具有显著性）。肝细胞癌病灶位于右叶时，PDCD4的平均值最高（P<0.05，具有显著性）。就分化程度而言，Ⅱ级分化的细胞色素c平均值更高（P<0.05，具有显著性）。
肝硬化和肝细胞癌患者血清中细胞色素c和PDCD4水平升高，可作为肝细胞癌的诊断辅助指标。
