Ashraf Rizwan, Sultana Bushra, Iqbal Munawar, Mushtaq Muhammad
Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan.
Department of Chemistry, The University of Lahore, Lahore, Pakistan.
J Genet Eng Biotechnol. 2016 Jun;14(1):1-8. doi: 10.1016/j.jgeb.2016.08.003. Epub 2016 Sep 10.
The present study was aimed to appraise the variations in biochemical, antioxidant and nutritional attributes of radish ( L.) under foliar application of plant leaf aqueous extracts i.e., mulberry leaf extract (MLE), brassica leaf extract (BLE), sorghum leaf extract (SLE) and moringa leaf extract (MoLE) as natural growth regulators. Samples were collected after three sprays of extracts and analyzed. Total phenolic constituents were determined using Folin-Ciocalteu reagent method, whereas antioxidant potential was evaluated by 1,1 diphenyl-2-picrylhydrazyl radical radical scavenging and reducing power assays. Results revealed that application of MLE, BLE, SLE, and MoLE not only improved growth, but also enhanced biochemical and antioxidant activities. Foliar spray of MoLE furnished relatively three folds higher amounts of extractable bioactive compounds (37.65 ± 0.94%), phenolic constituents (54.51-182.71 mg GAE/g f.w). The radical scavenging capacity (RSC) and reducing potential were also enhanced considerably. Furthermore, the moisture, dietary fiber, crude protein, and carotenoids were also enhanced in response of foliar spray of plant extracts. From results, it is concluded that plant extracts are effective sources of natural growth regulars and might be useful for the production of vegetables with improved nutritional value and antioxidant activity.
本研究旨在评估在叶面喷施桑树叶片水提取物（MLE）、芸苔叶片提取物（BLE）、高粱叶片提取物（SLE）和辣木叶片提取物（MoLE）作为天然生长调节剂的情况下，萝卜（L.）的生化、抗氧化和营养特性的变化。在喷施三次提取物后采集样本并进行分析。总酚成分采用福林-酚试剂法测定，抗氧化能力通过1,1-二苯基-2-苦基肼自由基清除和还原能力测定进行评估。结果表明，喷施MLE、BLE、SLE和MoLE不仅促进了生长，还增强了生化和抗氧化活性。叶面喷施MoLE提供了相对高三倍的可提取生物活性化合物（37.65±0.94%）、酚类成分（54.51 - 182.71毫克没食子酸当量/克鲜重）。自由基清除能力（RSC）和还原能力也显著增强。此外，植物提取物叶面喷施还提高了萝卜的水分、膳食纤维、粗蛋白和类胡萝卜素含量。从结果可以得出结论，植物提取物是天然生长调节剂的有效来源，可能有助于生产具有更高营养价值和抗氧化活性的蔬菜。
