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红冬孢菌素-6 通过激活阿片受体增强骨骼肌葡萄糖摄取。

Rubiscolin-6 activates opioid receptors to enhance glucose uptake in skeletal muscle.

机构信息

Department of Psychosomatic Internal Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.

Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia.

出版信息

J Food Drug Anal. 2019 Jan;27(1):266-274. doi: 10.1016/j.jfda.2018.06.012. Epub 2018 Aug 14.

DOI:10.1016/j.jfda.2018.06.012
PMID:30648580
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9298623/
Abstract

Rubiscolin-6 is an opioid peptide derived from plant ribulose bisphosphate carboxylase/oxygenase (Rubisco). It has been demonstrated that opioid receptors could control glucose homeostasis in skeletal muscle independent of insulin action. Therefore, Rubiscolin-6 may be involved in the control of glucose metabolism. In the present study, we investigated the effect of rubiscolin-6 on glucose uptake in skeletal muscle. Rubiscolin-6-induced glucose uptake was measured using the fluorescent indicator 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG) in L6 and C2C12 cell lines. The protein expressions of glucose transporter 4 (GLUT4) and AMP-activated protein kinase (AMPK) in L6 cells were observed by Western blotting. The in vivo effects of rubiscolin-6 were characterized in streptozotocin (STZ)-induced diabetic rats. Rubiscolin-6 induced a concentration-dependent increase in glucose uptake levels. The increase of phospho-AMPK (pAMPK) and GLUT4 expressions were also observed in L6 and C2C12 cells. Effects of rubiscolin-6 were blocked by opioid receptor antagonists and/or associated signals inhibitors. Moreover, Rubiscolin-6 produced a dose-dependent reduction of blood glucose and increased GLUT4 expression in STZ-induced diabetic rats. In conclusion, rubiscolin-6 increases glucose uptake, potentially via an activation of AMPK to enhance GLUT4 translocation after binding to opioid receptors in skeletal muscle.

摘要

Rubiscolin-6 是一种源自植物核酮糖二磷酸羧化酶/加氧酶（Rubisco）的阿片肽。已经证明，阿片受体可以独立于胰岛素作用控制骨骼肌中的葡萄糖稳态。因此，Rubiscolin-6 可能参与葡萄糖代谢的控制。在本研究中，我们研究了 Rubiscolin-6 对骨骼肌葡萄糖摄取的影响。Rubiscolin-6 诱导的葡萄糖摄取通过荧光指示剂 2-[N-(7-硝基苯并-2-氧代-1,3-二唑-4-基)氨基]-2-脱氧葡萄糖（2-NBDG）在 L6 和 C2C12 细胞系中进行测量。通过 Western blot 观察 L6 细胞中葡萄糖转运蛋白 4 (GLUT4) 和 AMP 激活蛋白激酶 (AMPK) 的蛋白表达。Rubiscolin-6 的体内作用在链脲佐菌素（STZ）诱导的糖尿病大鼠中进行了表征。Rubiscolin-6 诱导葡萄糖摄取水平呈浓度依赖性增加。还观察到 L6 和 C2C12 细胞中磷酸化 AMPK (pAMPK) 和 GLUT4 表达的增加。Rubiscolin-6 的作用被阿片受体拮抗剂和/或相关信号抑制剂阻断。此外，Rubiscolin-6 可降低 STZ 诱导的糖尿病大鼠的血糖水平并增加 GLUT4 表达。总之，Rubiscolin-6 通过与骨骼肌中的阿片受体结合，增加葡萄糖摄取，可能通过激活 AMPK 来增强 GLUT4 易位。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/4ec82506c9cf/jfda-27-01-266f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/065196d3ee44/jfda-27-01-266f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/202d8c293083/jfda-27-01-266f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/ec6bb09fc95c/jfda-27-01-266f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/4ec82506c9cf/jfda-27-01-266f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/065196d3ee44/jfda-27-01-266f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/202d8c293083/jfda-27-01-266f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/ec6bb09fc95c/jfda-27-01-266f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8833/9298623/4ec82506c9cf/jfda-27-01-266f5.jpg

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本文引用的文献

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2
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Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):101-106. doi: 10.1016/j.bbrc.2017.10.075. Epub 2017 Oct 16.
3
Review of Opioid Pharmacogenetics and Considerations for Pain Management.阿片类药物药物遗传学评价及疼痛管理注意事项。
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Food Chem X. 2023 Mar 13;18:100640. doi: 10.1016/j.fochx.2023.100640. eCollection 2023 Jun 30.
4
Rubiscolin‑6 rapidly suppresses the postprandial motility of the gastric antrum and subsequently increases food intake via δ‑opioid receptors in mice.瑞布索林-6 通过 δ-阿片受体在小鼠中快速抑制胃窦的餐后运动，随后通过 δ-阿片受体增加食物摄入。
Mol Med Rep. 2022 Nov;26(5). doi: 10.3892/mmr.2022.12856. Epub 2022 Sep 16.
5
Pepsin-digested chicken-liver hydrolysate attenuates hepatosteatosis by relieving hepatic and peripheral insulin resistance in long-term high-fat dietary habit.胃蛋白酶消化鸡肝水解物通过缓解肝脏和外周胰岛素抵抗减轻长期高脂肪饮食习惯引起的肝脂肪变性。
J Food Drug Anal. 2021 Jun 15;29(2):375-388. doi: 10.38212/2224-6614.3351.
6
Functional chicken-liver hydrolysates ameliorate insulin resistance and cognitive decline in streptozotocin-induced diabetic mice.功能性鸡肝水解物可改善链脲佐菌素诱导的糖尿病小鼠的胰岛素抵抗和认知功能下降。
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7
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4
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7
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8
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10
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