Department of Animal Science and Technology, National Taiwan University, Taipei City 106, Taiwan.
Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei City 106, Taiwan.
Poult Sci. 2022 Jun;101(6):101887. doi: 10.1016/j.psj.2022.101887. Epub 2022 Mar 31.
As part of the slaughtering processing in Taiwan, approximately 10,000 metric tons of broiler livers are produced yearly. However, these livers are regarded as waste. Our team has successfully developed a functional chicken-liver hydrolysate (CLH) with several useful activities. It has been reported that there is a positive relationship between diabetes mellitus (DM) patients and cognitive decline. To maximize broiler-livers' utilization and add value, we investigated the modulative effects of the CLHs on glucose homeostasis and cognitive decline in streptozotocin (STZ) induced diabetic mice. After a 9-wk experiment, CLH supplementation lowered blood glucose by increasing GLUT4 protein expressions in the brains, livers, and muscles of STZ-induced mice (P < 0.05). CLHs also enhanced antioxidant capacities in the livers and brains of STZ-induced mice. Amended memory and alternation behavior were tested by using water and Y-maze assays (P < 0.05). Besides, STZ-induced mice with CLH supplementation had less contracted neuron bodies in the hippocampus and lower (P < 0.05) Aβ depositions in the dentate gyrus area. Less AGE accumulation and apoptosis-related proteins (RAGE, JNK, and activated Caspase 3) in the brains of STZ-induced mice were also detected by supplementing CLHs (P < 0.05). In conclusion, the results from this study offer not only scientific evidence on the amelioration of insulin resistance and cognitive decline in hyperglycemia but also add value to this byproduct.
在台湾的屠宰加工过程中，每年大约生产 1 万吨肉鸡肝脏。然而，这些肝脏被视为废物。我们的团队已经成功开发出一种具有多种有用活性的功能性鸡肝水解物（CLH）。据报道，糖尿病（DM）患者与认知能力下降之间存在正相关关系。为了最大限度地利用肉鸡肝脏并增加其价值，我们研究了 CLH 对链脲佐菌素（STZ）诱导的糖尿病小鼠葡萄糖稳态和认知能力下降的调节作用。经过 9 周的实验，CLH 补充剂通过增加大脑、肝脏和肌肉中的 GLUT4 蛋白表达降低了 STZ 诱导的小鼠的血糖（P<0.05）。CLH 还增强了 STZ 诱导的小鼠肝脏和大脑中的抗氧化能力。通过使用水迷宫和 Y 迷宫测试（P<0.05）来评估改善的记忆和交替行为。此外，补充 CLH 后，STZ 诱导的小鼠海马体中神经元体收缩减少，齿状回区 Aβ 沉积减少（P<0.05）。补充 CLH 还可以检测到大脑中 AGE 积累和与凋亡相关的蛋白质（RAGE、JNK 和活化的 Caspase 3）减少（P<0.05）。总之，本研究结果不仅为高血糖症中胰岛素抵抗和认知能力下降的改善提供了科学依据，而且还为这种副产品增加了价值。
