Exercise Oncology Research Laboratory, Department of Exercise and Sport Science, University of North Carolina at Chapel Hill, NC.
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC.
Med Sci Sports Exerc. 2019 Feb;51(2):379-388. doi: 10.1249/MSS.0000000000001780.
Mucosal-associated invariant T (MAIT) cells have properties of both the innate and adaptive immune systems but are an understudied population within exercise immunology. These lymphocytes aggregate at the mucous membranes, but it is unknown if submaximal exercise alters their circulating numbers or function.
To determine the MAIT cell response to submaximal exercise on activation and homing marker expression and stimulated cytokine production.
Twenty healthy, young, recreationally active males cycled for 40 min at 86% of VT after an overnight fast. Peripheral blood mononuclear cells were isolated and labeled to identify specific MAIT cell populations using flow cytometry. Cytokine production after stimulation was also determined.
Mucosal-associated invariant T cells were 2.9% of T cells and increased to 3.9% after exercise and with recovery whereas cell numbers significantly increased by 91.5% after exercise before returning to resting levels. Chemokine and activation marker absolute cell number significantly increased while expression levels remained constant but the high levels of CCR5 may help direct MAIT cells to sites of inflammation. After stimulation, TNFα expression significantly increased after exercise before returning to baseline with a similar trend for IFNγ.
The MAIT cell numbers undergo a partial biphasic response after submaximal exercise and appear to be preferentially mobilized within T cells; however, the magnitude of the submaximal response was attenuated relative to maximal exercise. Stimulated MAIT cells increase TNFα expression, indicating greater responsiveness to pathogens after acute exercise.
黏膜相关不变 T（MAIT）细胞具有先天和适应性免疫系统的特性，但在运动免疫学中研究不足。这些淋巴细胞聚集在黏膜处，但尚不清楚亚最大运动是否会改变它们的循环数量或功能。
确定 MAIT 细胞对亚最大运动的反应，包括激活和归巢标志物表达以及刺激细胞因子产生。
20 名健康、年轻、有规律运动的男性在禁食一夜后以 86%的 VT 进行 40 分钟的自行车运动。分离外周血单核细胞并进行标记，使用流式细胞术鉴定特定的 MAIT 细胞群体。还测定了刺激后的细胞因子产生。
黏膜相关不变 T 细胞占 T 细胞的 2.9%，运动后增加到 3.9%，并在恢复时增加，而细胞数量在运动后显著增加了 91.5%，然后在恢复到静息水平之前恢复到静息水平。趋化因子和激活标志物的绝对细胞数显著增加，而表达水平保持不变，但高水平的 CCR5 可能有助于将 MAIT 细胞导向炎症部位。运动后，TNFα 的表达显著增加，然后在恢复到基线之前，IFNγ 的表达也呈相似趋势。
MAIT 细胞数量在亚最大运动后经历部分双相反应，似乎优先在 T 细胞中动员；然而，亚最大运动的反应幅度相对于最大运动减弱。刺激后的 MAIT 细胞增加 TNFα 的表达，表明急性运动后对病原体的反应性增强。
