确定芽囊原虫的亚型并评估这些亚型对致病性的影响。
Determination the Subtypes of Blastocystis sp. and Evaluate the Effect of These Subtypes on Pathogenicity.
作者信息
Cakir Fatih, Cicek Mutalip, Yildirim Ibrahim Halil
机构信息
Department of Microbiology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
Department of Microbiology, Faculty of Medicine, Ahi Evran University, Kirsehir, Turkey.
出版信息
Acta Parasitol. 2019 Mar;64(1):7-12. doi: 10.2478/s11686-018-00002-y. Epub 2019 Jan 16.
PURPOSE
The present study aimed to determine the frequency of subtypes of Blastocystis sp. in the immunosuppressed individuals, in patients with chronic urticaria, and in patients with GIS complaints to investigate the difference of Blastocystis sp. subtype distribution between patient and control groups.
METHODS
A total of 345 stool samples were collected from the patients and samples were studied by native-Lugol, trichrome staining, and Jones medium culture method. Positively detected samples were subjected to PCR to determine the subtypes.
RESULTS
This is the first study of nine subtypes of Blastocystis sp. investigated in our country and the most frequently found subtype was ST3, and then, the other subtypes were ST1, ST2, ST5, and ST6, respectively. Mix subtype was detected in the 11.6% and no subtype was detected in the 17.4% of the samples. The ST5 was detected first time in the control group and ST6, which is reported limitedly in our country, was found in patients with GIS complaints. ST1 and ST2 were found higher in the patient group.
CONCLUSION
This study confirmed that the subtype (ST) differences are an important factor affecting the pathogenesis of Blastocystis sp.
目的
本研究旨在确定免疫抑制个体、慢性荨麻疹患者以及胃肠道症状（GIS）患者中芽囊原虫（Blastocystis sp.）亚型的频率，以调查患者组和对照组之间芽囊原虫亚型分布的差异。
方法
从患者中总共收集了345份粪便样本，并通过自然卢戈氏碘液染色、三色染色和琼斯培养基培养法对样本进行研究。对检测呈阳性的样本进行聚合酶链反应（PCR）以确定亚型。
结果
这是我国首次对芽囊原虫的九种亚型进行研究，最常见的亚型是ST3，其次是ST1、ST2、ST5和ST6。在11.6%的样本中检测到混合亚型，17.4%的样本未检测到亚型。在对照组中首次检测到ST5，在有胃肠道症状的患者中发现了我国报道较少的ST6。ST1和ST2在患者组中出现的比例更高。
结论
本研究证实亚型（ST）差异是影响芽囊原虫发病机制的重要因素。
Zotero 插件