Shi Xinge, Chen Xin
Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China.
Department of Cardiovascular Surgery, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Exp Ther Med. 2019 Jan;17(1):115-122. doi: 10.3892/etm.2018.6961. Epub 2018 Nov 13.
As a global health problem, cardiovascular disease threatens the lives of human beings. It has been reported that microRNAs (miRs) are important in regulating coronary atherosclerosis. In the present study, the expression levels of miR-370 in peripheral blood mononuclear cells of patients with coronary atherosclerosis were significantly increased compared with healthy patients, as demonstrated by reverse transcription-quantitative polymerase chain reaction analysis. Additionally, the target of miR-370 was predicted as Forkhead Box 1 (FOXO1) with bioinformatics, and was confirmed by a dual luciferase assay. The mRNA and protein expression levels of FOXO1 were inhibited by miR-370. Furthermore, the invasion and proliferation of human umbilical vein endothelial cells were promoted by miR-370 via inhibiting the expression of FOXO1. The results obtained in the present study demonstrated that miR-370 served an important role in regulating coronary atherosclerosis via targeting FOXO1. The present data also indicated that miR-370 may be a promising molecular target for treating coronary atherosclerosis.
作为一个全球性的健康问题，心血管疾病威胁着人类的生命。据报道，微小RNA（miRs）在调节冠状动脉粥样硬化中起重要作用。在本研究中，通过逆转录-定量聚合酶链反应分析表明，与健康患者相比，冠状动脉粥样硬化患者外周血单个核细胞中miR-370的表达水平显著升高。此外，通过生物信息学预测miR-370的靶标为叉头框蛋白1（FOXO1），并通过双荧光素酶报告基因检测得到证实。miR-370抑制了FOXO1的mRNA和蛋白表达水平。此外，miR-370通过抑制FOXO1的表达促进了人脐静脉内皮细胞的侵袭和增殖。本研究获得的结果表明，miR-370通过靶向FOXO1在调节冠状动脉粥样硬化中起重要作用。本研究数据还表明，miR-370可能是治疗冠状动脉粥样硬化的一个有前景的分子靶点。
