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2型糖尿病患者循环外周血单个核细胞中凋亡蛋白的基因表达谱及二甲双胍的调节作用

Gene Expression Profiling of Apoptotic Proteins in Circulating Peripheral Blood Mononuclear Cells in Type II Diabetes Mellitus and Modulation by Metformin.

作者信息

Al Dubayee Mohammed, Alshahrani Awad, Aljada Dana, Zahra Mahmoud, Alotaibi Ahmed, Ababtain Ibrahim, Alnaim Malik, Alahmari Ali, Aljarallah Abdullah, Elahi Muhammad Affan, Fakhoury Hana M A

机构信息

College of Medicine, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia.

King Abdullah International Medical Research Center (KAIMRC), Riyadh, Saudi Arabia.

出版信息

Diabetes Metab Syndr Obes. 2021 Mar 15;14:1129-1139. doi: 10.2147/DMSO.S300048. eCollection 2021.

DOI:10.2147/DMSO.S300048
PMID:33758522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7979348/
Abstract

INTRODUCTION

Insulin resistance in obesity and type 2 diabetes mellitus (T2DM) is associated with cardiovascular complications such as atherosclerosis. On the other hand, the reduction of apoptosis in macrophages has been linked with accelerated atherosclerosis. Apoptosis is controlled by a different family of proteins including Bcl-2 and caspases.

METHODS

To examine apoptosis in insulin resistance, we assessed the mRNA expression by qRT-PCR of several Bcl-2 family members, as well as caspase-3, -7, -8, and -9 in peripheral blood mononuclear cells (PBMCs) isolated from lean, obese, diabetic, and diabetic on metformin individuals.

RESULTS

PBMCs of diabetic individuals exhibited reduced expression of caspase-7 and increased expression of Bcl-10, Bad, Bax, Bid, and caspase-3. T2DM on metformin group had significantly higher Bad, Bax, and caspase-7 expression.

DISCUSSION

The moderate up-regulation of pro-apoptotic Bcl-10, Bax, Bad, Bid, and the effector caspase-3 coupled with inhibition of caspase-7 in circulating PBMCs of T2DM could be the result of increased inflammation in T2DM. Metformin treatment significantly inhibited the expression of Bcl-10, Bid, and caspase-3 and upregulated Bad/Bax/caspase-7 pathway suggesting the activation of Bad/Bax/caspase-7 apoptotic pathway. Further studies are warranted to elicit the underlying apoptotic pathways of PBMCs in T2DM and following metformin treatment.

摘要

引言

肥胖和2型糖尿病（T2DM）中的胰岛素抵抗与动脉粥样硬化等心血管并发症相关。另一方面，巨噬细胞凋亡的减少与动脉粥样硬化加速有关。凋亡由包括Bcl-2和半胱天冬酶在内的不同蛋白质家族控制。

方法

为了研究胰岛素抵抗中的凋亡，我们通过qRT-PCR评估了从瘦人、肥胖者、糖尿病患者以及服用二甲双胍的糖尿病患者中分离出的外周血单核细胞（PBMC）中几种Bcl-2家族成员以及半胱天冬酶-3、-7、-8和-9的mRNA表达。

结果

糖尿病患者的PBMC显示半胱天冬酶-7表达降低，而Bcl-10、Bad、Bax、Bid和半胱天冬酶-3表达增加。服用二甲双胍的T2DM组中Bad、Bax和半胱天冬酶-7的表达显著更高。

讨论

T2DM循环PBMC中促凋亡的Bcl-10、Bax、Bad、Bid以及效应半胱天冬酶-3的适度上调与半胱天冬酶-7的抑制可能是T2DM中炎症增加的结果。二甲双胍治疗显著抑制Bcl-10、Bid和半胱天冬酶-3的表达，并上调Bad/Bax/半胱天冬酶-7途径，提示Bad/Bax/半胱天冬酶-7凋亡途径的激活。有必要进行进一步研究以阐明T2DM及二甲双胍治疗后PBMC潜在的凋亡途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/7ad56ec9489e/DMSO-14-1129-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/a3e56afb1c54/DMSO-14-1129-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/952a001f3b3c/DMSO-14-1129-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/b7625ce3a498/DMSO-14-1129-g0003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/fabebe7ff60a/DMSO-14-1129-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/7ad56ec9489e/DMSO-14-1129-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/a3e56afb1c54/DMSO-14-1129-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/952a001f3b3c/DMSO-14-1129-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/b7625ce3a498/DMSO-14-1129-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/8c993ea926f6/DMSO-14-1129-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/fabebe7ff60a/DMSO-14-1129-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f31/7979348/7ad56ec9489e/DMSO-14-1129-g0006.jpg

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本文引用的文献

1
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Cell Biol Int. 2020 Aug;44(8):1628-1639. doi: 10.1002/cbin.11355. Epub 2020 Apr 18.
2
BCL10 in cell survival after DNA damage.BCL10 在 DNA 损伤后的细胞存活中发挥作用。
Clin Chim Acta. 2019 Aug;495:301-308. doi: 10.1016/j.cca.2019.04.077. Epub 2019 Apr 29.
3
Effect of microRNA-370 on coronary atherosclerosis and its underlying mechanism.微小RNA-370对冠状动脉粥样硬化的影响及其潜在机制。
Exp Ther Med. 2019 Jan;17(1):115-122. doi: 10.3892/etm.2018.6961. Epub 2018 Nov 13.
4
Metformin protects against oxidized low density lipoprotein-induced macrophage apoptosis and inhibits lipid uptake.二甲双胍可防止氧化型低密度脂蛋白诱导的巨噬细胞凋亡并抑制脂质摄取。
Exp Ther Med. 2018 Mar;15(3):2485-2491. doi: 10.3892/etm.2018.5704. Epub 2018 Jan 5.
5
Global aetiology and epidemiology of type 2 diabetes mellitus and its complications.2 型糖尿病及其并发症的全球病因学和流行病学。
Nat Rev Endocrinol. 2018 Feb;14(2):88-98. doi: 10.1038/nrendo.2017.151. Epub 2017 Dec 8.
6
Bad phosphorylation as a target of inhibition in oncology.不良磷酸化作为肿瘤抑制的靶点。
Cancer Lett. 2018 Feb 28;415:177-186. doi: 10.1016/j.canlet.2017.11.017. Epub 2017 Nov 23.
7
Cell Death in the Vessel Wall: The Good, the Bad, the Ugly.血管壁细胞死亡：好、坏、丑。
Arterioscler Thromb Vasc Biol. 2017 Jul;37(7):e75-e81. doi: 10.1161/ATVBAHA.117.309229.
8
Leukocyte Bim deficiency does not impact atherogenesis in ldlr mice, despite a pronounced induction of autoimmune inflammation.白细胞 Bim 缺乏症不会影响 LDLR 小鼠的动脉粥样硬化形成，尽管其明显诱导了自身免疫炎症。
Sci Rep. 2017 Jun 8;7(1):3086. doi: 10.1038/s41598-017-02771-4.
9
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Oxid Med Cell Longev. 2016;2016:3087469. doi: 10.1155/2016/3087469. Epub 2016 Oct 26.
10
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