Sutherland Christina A, Ozbal Can, Nicolau David P
Center for Anti-Infective Research & Development Hartford Hospital, Hartford, CT, 06102 USA.
PureHoney Technologies, Inc., Billerica, MA, 01821 USA.
Future Sci OA. 2018 Nov 15;5(1):FSO352. doi: 10.4155/fsoa-2018-0079. eCollection 2019 Jan.
We describe the validation of an HPLC-MS/MS method to analyze ceftolozane and tazobactam simultaneously in saline matrixes.
MATERIALS & METHODS: An Agilent 1260 HPLC interfaced to an Agilent 6470 triple-quadrupole mass spectrometer was used for quantification. A reverse-phase column running a gradient of water and acetonitrile containing 0.1% formic acid mobile phase at a flow rate of 1.0 ml/min provided chromatographic fractionation. TazobactamN was used as the internal standard. The standard curves were linear over a range of 0.02-0.5 μg/ml.
This methodology represents a simple, reproducible approach to the determination of drug concentrations with accuracy and precision for pharmacokinetic studies undertaken with this recently US FDA-approved antimicrobial therapy.
我们描述了一种用于在盐溶液基质中同时分析头孢洛扎和他唑巴坦的高效液相色谱-串联质谱法（HPLC-MS/MS）的验证。
使用与安捷伦6470三重四极杆质谱仪联用的安捷伦1260高效液相色谱仪进行定量分析。采用反相柱，以含0.1%甲酸的水和乙腈为流动相进行梯度洗脱，流速为1.0毫升/分钟，实现色谱分离。使用他唑巴坦N作为内标。标准曲线在0.02 - 0.5微克/毫升范围内呈线性。
这种方法代表了一种简单、可重复的方法，可准确、精确地测定药物浓度，用于美国食品药品监督管理局（FDA）最近批准的这种抗菌治疗的药代动力学研究。
