七酶体外级联反应生成 (3R)-3-羟基丁酰-CoA。
Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA.
机构信息
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
出版信息
Org Biomol Chem. 2019 Feb 6;17(6):1375-1378. doi: 10.1039/c8ob02858c.
Abstract
Economical and environmentally-friendly routes to convert feedstock chemicals like acetate into valuable chiral products such as (R)-3-hydroxybutyrate are in demand. Here, seven enzymes (CoaA, CoaD, CoaE, ACS, BktB, PhaB, and GDH) are employed in a one-pot, in vitro, biocatalytic synthesis of (3R)-3-hydroxybutyryl-CoA, which was readily isolated. This platform generates not only chiral diketide building blocks but also desirable CoA derivatives.
摘要
人们需要经济环保的方法，将乙酸盐等原料化学品转化为有价值的手性产物，如（R）-3-羟基丁酸。在这里，七种酶（CoaA、CoaD、CoaE、ACS、BktB、PhaB 和 GDH）被用于一锅、体外的生物催化合成（3R）-3-羟丁酰辅酶 A，该物质很容易被分离出来。该平台不仅生成手性二酮酸基砌块，还生成有价值的辅酶 A 衍生物。
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