Quin M B, Wallin K K, Zhang G, Schmidt-Dannert C
University of Minnesota, Dept. of Biochemistry, Molecular Biology and Biophysics, 1479 Gortner Avenue, St Paul, MN 55108, USA.
Org Biomol Chem. 2017 May 23;15(20):4260-4271. doi: 10.1039/c7ob00391a.
Industrial biocatalysis is an economically attractive option for the production of valuable chemicals. Our repertoire of cheap building blocks and commodity target molecules is vastly enhanced by multi-enzyme biocatalytic cascades. In order to achieve suitable titers in complex novel biocatalytic schemes, spatial organization may become necessary to overcome barriers caused by slow or inhibited enzymes as well as instability of biocatalysts. A number of spatial organization strategies are currently available, which could be integrated in the design of complex cascades. These include fusion proteins, immobilization on solid supports, multi-dimensional scaffolding, and encapsulation within vessels. This review article highlights recent advances in cascade biocatalysis, discusses the role of spatial organization in reaction kinetics, and presents some of the currently employed strategies for spatial organization of multi-enzyme cascades.
工业生物催化是生产有价值化学品的一种具有经济吸引力的选择。多酶生物催化级联反应极大地扩展了我们廉价基础原料和商品目标分子的种类。为了在复杂的新型生物催化方案中获得合适的滴度，可能需要进行空间组织以克服由酶促反应缓慢或受抑制以及生物催化剂不稳定所造成的障碍。目前有多种空间组织策略可供使用，这些策略可整合到复杂级联反应的设计中。这些策略包括融合蛋白、固定在固体载体上、多维支架构建以及封装在容器中。本文综述了级联生物催化的最新进展，讨论了空间组织在反应动力学中的作用，并介绍了目前用于多酶级联反应空间组织的一些策略。
