Marker Assisted Selection in Sorghum Using KASP Assay for the Detection of Single Nucleotide Polymorphism/Insertion Deletion.

Marker assisted selection (MAS), an advance tool in plant breeding that allows accurate and efficient introgression of important agronomic trait(s) from a germplasm source to desired elite lines, has been applied to sorghum recently. Here, we report the methods for the deployment of MAS for trait introgression using endpoint genotyping technology for single nucleotide polymorphism (SNP)/insertion deletion (InDel) coupled with an application of KASP (Kompetitive Allele Specific Polymerase Chain Reaction [PCR]) chemistry allowing for the selection of parents for generational advancement without going through the laborious and time consuming phenotypic selection and additional generations for selection of desired individuals. This MAS-SNP marker assisted backcrossing scheme can be applied to accurately select heterozygotes for use as an allele donor parent in each backcross generation, thus expediting the backcrossing scheme and resulting in time savings of 3 years compared to conventional methods of introgression practiced in sorghum breeding and improvement.