Monteiro Babyla Geraldes, Loureiro Renata Ruoco, Cristovam Priscila Cardoso, Covre Joyce Luciana, Gomes José Álvaro Pereira, Kerkis Irina
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
Departamento de Morfologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
Arq Bras Oftalmol. 2019 Jan-Feb;82(1):32-37. doi: 10.5935/0004-2749.20190009.
To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes.
We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components.
Immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro.
Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction.
评估源自神经嵴的间充质干细胞——人未成熟牙髓干细胞，在去上皮羊膜上培养时分化为角膜上皮用于角膜移植和组织工程的能力。
我们比较了在无血清条件下或含有血清或血清替代成分的培养基中，在羊膜或塑料表面生长的细胞的免疫表型（ABCG2、K3/12和波形蛋白）。
在基础条件下在羊膜上生长的未成熟牙髓干细胞能够维持其未分化状态。我们的数据还表明，本研究中使用的培养基可以调节未成熟牙髓干细胞标志物的表达，从而在体外诱导这些细胞的上皮分化。
我们的结果表明，在临床眼表重建中，羊膜是间充质干细胞尤其是未成熟牙髓干细胞生长和移植的良好选择。
