Kayser K, Stute H, Lübcke J, Wazinski U
Department of Pathology, Hospital for Thoracic Diseases, Heidelberg, FR Germany.
Histochem J. 1988 Jun-Jul;20(6-7):347-52. doi: 10.1007/BF01002728.
Tissue blocks taken from healthy human lung tissues, from primary bronchial carcinoma and from mediastinal and hilar lymph nodes were placed in the following solutions: Tris buffer; buffered formalin (0.5%, 1%, 7%); 0.1 mol NaCl; distilled water; DMSO (1%, 10%, 20%); acetone (10%); methanol (50%, 80%, 100%); glutaraldehyde (2.5%), and fixed by use of a commercial microwave oven. Tissue blocks obtained from the same surgical specimens were fixed in 7% buffered formalin for 24 h for comparison. Conventional and microwave-fixed tissue was embedded in paraffin, and stained with haematoxylin and eosin. Fifteen specimens of each group and each solution were examined by light microscopy. Minimum diameter and area of 100 nuclei of each specimen were measured interactively. Histomorphological sections fixed with Tris buffer in a microwave oven revealed best morphological results showing more contrast in chromatin distribution of nuclei and 'opening' of interstitial lung capillaries in comparison to conventional formalin-fixed specimens. No statistically significant differences in area and minimum diameter of nuclei between the different groups were found. Microwave fixation using Tris buffer is a time-saving fixation method at least comparable to conventional formalin fixation. It is not accompanied by hazards to the environment that are unavoidable by formalin fixation.
取自健康人肺组织、原发性支气管癌以及纵隔和肺门淋巴结的组织块被置于以下溶液中：Tris缓冲液；缓冲福尔马林（0.5%、1%、7%）；0.1 mol氯化钠；蒸馏水；二甲基亚砜（1%、10%、20%）；丙酮（10%）；甲醇（50%、80%、100%）；戊二醛（2.5%），并使用商用微波炉进行固定。从相同手术标本获取的组织块用7%缓冲福尔马林固定24小时以作比较。常规固定和微波固定的组织均用石蜡包埋，并用苏木精和伊红染色。每组每种溶液的15个标本通过光学显微镜检查。交互式测量每个标本100个细胞核的最小直径和面积。与传统福尔马林固定标本相比，在微波炉中用Tris缓冲液固定的组织形态学切片显示出最佳的形态学结果，细胞核染色质分布对比度更高，肺间质毛细血管“开放”。不同组之间细胞核面积和最小直径未发现统计学上的显著差异。使用Tris缓冲液的微波固定是一种省时的固定方法，至少与传统福尔马林固定相当。它不会像福尔马林固定那样带来不可避免的环境危害。
