Mac-Moune Lai F, Lai K N, Chew E C, Lee J C
Pathology. 1987 Jan;19(1):17-21. doi: 10.3109/00313028709065129.
Microwave-fixed tissues were examined in 10 patients undergoing diagnostic renal biopsy. A small portion of renal tissue was fixed by microwave irradiation and subsequently processed by routine methods for light microscopic, immunofluorescent and electron microscopic studies. The remaining portion of specimen was fixed and processed by conventional methods. In light microscopic examination, the renal architecture and cell morphology were well-preserved. Pathological changes were identical to those seen with formalin-fixed tissue. The pattern, distribution and intensity of positive immunofluorescence in microwave-fixed tissue were similar to those in tissues directly snap-frozen and stained. In electron microscopy, the normal and pathological features were well-demonstrated and not different from those observed in glutaraldehyde-fixed specimens. Specific ultrastructural lesions were clearly demonstrated and, apparently, were not altered by microwave irradiation. Our preliminary data indicate that microwave fixation can be effectively applied in the processing of renal biopsies. As the fixation is rapid, this method may be valuable in circumstances when an urgent diagnosis is required.
对10例接受诊断性肾活检的患者的微波固定组织进行了检查。取一小部分肾组织经微波照射固定，随后采用常规方法进行光镜、免疫荧光和电镜研究。标本的其余部分采用传统方法固定和处理。在光镜检查中，肾脏结构和细胞形态保存良好。病理变化与福尔马林固定组织所见相同。微波固定组织中阳性免疫荧光的模式、分布和强度与直接速冻和染色的组织相似。在电子显微镜下，正常和病理特征得到了很好的显示，与戊二醛固定标本中观察到的特征没有差异。特定的超微结构病变清晰可见，显然未因微波照射而改变。我们的初步数据表明，微波固定可有效地应用于肾活检组织的处理。由于固定迅速，该方法在需要紧急诊断的情况下可能具有重要价值。
