Van Dort J B, De Bruijn W C, Schneijdenberg C T, Boon M E, Kok L P
Hogeschool West-Brabant, Department of Histology and Cytology, Etten-Leur, The Netherlands.
Histochem J. 1988 Jun-Jul;20(6-7):365-72. doi: 10.1007/BF01002731.
The application of microwave irradiation at various steps in 'normal' EM procedures has been investigated. 1. The temperatures in various aqueous volumes were measured during microwave irradiation. Increases were small and the final temperature could be controlled by cooling the glass base plate. 2. The influence of microwave irradiation on the various fixation schedules for the electron microscopy of single cell populations was studied and the results favoured the idea that by the application of microwave irradiation a more life-like ultrastructural preservation could be obtained. 3. Peroxidase-like activity in erythrocytes, acid phosphatase activity in resident macrophages and peroxidase activity in monocyte granules was apparently not influenced by microwave irradiation during aldehyde fixation and incubation. 4. The anticipated microwave-induced penetration enhancement of cerium ions in a cytochemical reaction procedure to detect acid phosphatase activity in resident macrophages was not observed.
已经研究了在“常规”电子显微镜程序的各个步骤中微波辐射的应用。1. 在微波辐射期间测量了不同体积水溶液中的温度。温度升高幅度较小，最终温度可通过冷却玻璃基板来控制。2. 研究了微波辐射对单细胞群体电子显微镜观察的各种固定方案的影响，结果支持这样一种观点，即通过应用微波辐射可以获得更逼真的超微结构保存。3. 在醛固定和孵育期间，红细胞中的过氧化物酶样活性、驻留巨噬细胞中的酸性磷酸酶活性以及单核细胞颗粒中的过氧化物酶活性显然不受微波辐射的影响。4. 在检测驻留巨噬细胞中酸性磷酸酶活性的细胞化学反应过程中，未观察到预期的微波诱导的铈离子渗透增强。
