Rosenquist M D, Kealey G P, Lewis R W, Cram A E
University of Iowa Burn Treatment Center, Iowa City 52242.
J Burn Care Rehabil. 1988 Nov-Dec;9(6):634-6. doi: 10.1097/00004630-198811000-00013.
Skin stored in nutrient medium at 4 degrees C produces acceptable short-term viability. This study compared the storage viability of nonmeshed v meshed skin stored at 4 degrees C in nutrient medium. Skin specimens from six human donors were stored for up to 35 days in RPMI 1640 tissue culture medium at 4 degrees C. Skin specimens (1 cm in diameter) were transplanted to surgically created defects on the thorax of nude mice at fixed intervals during the storage period. Gross and microscopic techniques were used to determine the graft viability at 10 days postgraft. Skin was divided into two storage groups as nonmeshed or meshed 1.5/1. The storage configuration was free-floating, 10 cm x 2 cm sheet grafts. The ratio of skin surface area to volume medium was 300 cm2/100 mL. There was no significant difference between the viability of the nonmeshed group compared to the meshed group. Prior meshing of human allograft does not adversely affect the viability of banked skin. Therefore, skin can be stored in a meshed configuration. This eliminates operating room time spent preparing allograft for application, which is cost-effective.
储存在4摄氏度营养培养基中的皮肤具有可接受的短期活力。本研究比较了在4摄氏度营养培养基中储存的非网状皮肤与网状皮肤的储存活力。来自六名人类供体的皮肤样本在4摄氏度的RPMI 1640组织培养基中储存长达35天。在储存期间，每隔固定时间将直径1厘米的皮肤样本移植到裸鼠胸部手术造成的缺损处。在移植后10天，使用大体和显微镜技术来确定移植物的活力。皮肤被分为非网状或1.5/1网状两个储存组。储存形态为自由漂浮的10厘米×2厘米片状移植物。皮肤表面积与培养基体积之比为300平方厘米/100毫升。非网状组与网状组的活力之间没有显著差异。人同种异体移植物预先网状化不会对储存皮肤的活力产生不利影响。因此，皮肤可以以网状形态储存。这消除了在手术室为应用准备同种异体移植物所花费的时间，具有成本效益。
