Rosenquist M D, Cram A E, Kealey G P
University of Iowa Burn Center, University of Iowa Hospitals and Clinics, Iowa City 52242.
Cryobiology. 1988 Feb;25(1):31-7. doi: 10.1016/0011-2240(88)90017-x.
There are numerous experimental studies in the literature regarding skin storage and preservation. These studies are difficult to interpret due to the variety of storage techniques utilized and the number of different animal species used as skin donors. This study utilized a single cold storage protocol to test the effect of species variation on skin graft viability. Donor skin was obtained from five animal species and human surgical panniculectomy specimens. The skin was stored in modified Roswell Park Memorial Institute (RPMI) 1640 tissue culture media at 4 degrees C. Stored skin was transplanted to surgically created defects on athymic (nude) mice after specific storage intervals. Ten days after transplantation, the grafts were examined by gross and microscopic techniques. The viability of mouse, rat, and dog skin was significantly different from human skin, while stored rabbit and pig skin were similar to human skin. These results demonstrate the difficulty of applying the data of skin storage studies from nonhuman species to clinical practice. The data indicate that rabbit and pig skin may be used in laboratory studies of skin preservation at 4 degrees C with a strong likelihood that the results may be of clinical relevance in predicting the behavior of human skin under similar storage conditions.
文献中有大量关于皮肤储存和保存的实验研究。由于所采用的储存技术种类繁多以及用作皮肤供体的不同动物物种数量众多，这些研究难以解读。本研究采用单一的冷藏方案来测试物种差异对皮肤移植存活率的影响。供体皮肤取自五个动物物种以及人类手术切除的腹壁脂肪标本。皮肤储存在改良的罗斯威尔帕克纪念研究所（RPMI）1640组织培养基中，温度为4摄氏度。在特定的储存时间间隔后，将储存的皮肤移植到无胸腺（裸）小鼠的手术创口上。移植十天后，通过大体和显微镜技术检查移植物。小鼠、大鼠和狗的皮肤存活率与人类皮肤有显著差异，而储存的兔和猪皮肤与人类皮肤相似。这些结果表明，将非人类物种的皮肤储存研究数据应用于临床实践存在困难。数据表明，兔和猪皮肤可用于4摄氏度下皮肤保存的实验室研究，很有可能其结果在预测人类皮肤在类似储存条件下的表现方面具有临床相关性。
