Rosenquist M D, Cram A E, Kealey G P
University of Iowa, Iowa City.
J Burn Care Rehabil. 1988 Jan-Feb;9(1):52-4. doi: 10.1097/00004630-198801000-00012.
This study was designed to examine the effect of the storage configuration of skin and the ratio of tissue-to-storage medium on the viability of skin stored under refrigeration. Human skin specimens were stored in four physical configurations in RPMI 1640 tissue culture media at 4 degrees C. Skin was transferred to surgically created defects on nude mice after specific storage intervals. Grafts were examined grossly and microscopically after ten days. In the rolled configuration, on storage day 15, the viability of the outside of the roll was significantly better than the inside (P less than 0.01). The graft viability of the outside of the skin rolls was similar for both tissue-to-media ratios as well as for both free-floating configurations (P = 0.27). These findings suggest the optimum cold storage configuration is free floating, and 300 cm2/100 mL is an appropriate skin surface area to volume media ratio. This proportion of tissue to media is in agreement with the minimum ratio currently recommended by the Skin Council of the American Association of Tissue Banks.
本研究旨在探讨皮肤的储存形态以及组织与储存介质的比例对冷藏保存皮肤活力的影响。将人体皮肤标本以四种物理形态保存在4℃的RPMI 1640组织培养基中。在特定的储存时间后，将皮肤移植到裸鼠手术造成的缺损处。十天后对移植片进行大体和显微镜检查。在卷状形态中，储存第15天时，卷外侧的活力明显优于内侧（P<0.01）。对于两种组织与介质比例以及两种漂浮形态，皮肤卷外侧的移植片活力相似（P = 0.27）。这些发现表明，最佳冷藏形态是漂浮，且300 cm2/100 mL是合适的皮肤表面积与培养基体积比。这种组织与介质的比例与美国组织库协会皮肤委员会目前推荐的最小比例一致。
