Chang P, Rosenquist M D, Lewis R W, Kealey G P
Department of Surgery, University of Iowa College of Medicine, Iowa City, USA.
J Burn Care Rehabil. 1998 Jan-Feb;19(1 Pt 1):25-8. doi: 10.1097/00004630-199801000-00007.
We sought to ascertain whether an in vitro assay could be as reliable as an in vivo assay in determining the viability of human skin stored at 4 degrees C. Allografts from six human donors were stored in RPMI 1640 tissue culture medium at 4 degrees C. At fixed intervals during the storage period, all skin specimens were tested concurrently by two different viability assays: (1) transplantation onto surgically created defects on nude mice, and (2) intracellular enzyme activity with use of a 4-hour semiquantitative micromethod system activity (API ZYM; Biomerieux Vitek Inc., Hazelwood, Mo.). Human graft survival on the nude mice was 100% for the first 15 days of storage, and then declined to 50% on storage day 30. The API ZYM assay showed a comparable progressive decrease in enzyme activity over skin storage time. The API ZYM assay is a simple, rapid system that produces reproducible results and is cost-effective when compared to the biologic model.
我们试图确定在测定4℃保存的人皮肤活力时，体外试验是否能与体内试验一样可靠。来自6名人类供体的同种异体移植物保存在RPMI 1640组织培养基中，温度为4℃。在保存期间的固定时间间隔，所有皮肤标本同时通过两种不同的活力试验进行检测：（1）移植到裸鼠手术造成的缺损处；（2）使用4小时半定量微量方法系统活性（API ZYM；法国生物梅里埃公司，密苏里州黑兹尔伍德）检测细胞内酶活性。在保存的前15天，裸鼠上的人移植物存活率为100%，然后在保存第30天时降至50%。API ZYM试验显示，随着皮肤保存时间的延长，酶活性呈类似的逐渐下降。与生物模型相比，API ZYM试验是一个简单、快速的系统，能产生可重复的结果，且成本效益高。
