Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.
College of pharmacy & Health Sciences, Ajman University, Ajman, United Arab Emirates.
Int J Biol Macromol. 2019 Apr 15;127:271-277. doi: 10.1016/j.ijbiomac.2019.01.055. Epub 2019 Jan 14.
Glutathione reductase (GR) is a flavoprotein that catalyses the reduction of oxidized glutathione (GSSG) to reduced glutathione (2GSH) in the presence of coenzyme NADPH. The importance of glutathione stems from the fact that it serves an important role in various metabolic processes. Plants growing in highly polluted areas are exposed to higher concentration of metal ions; thereby feeling abiotic stress and affecting various regulatory enzyme activities. In this study, effect of metal ions has been studied on GR. Phytocystatins show an increased expression in abiotic stress conditions. Here in, the effect of cystatin isolated from yellow mustard seeds (YMP) on heavy metals induced conformational changes in GR was investigated making use of GR activity assay, UV-absorption spectroscopy, fluorescence spectroscopy, FTIR, CD, ITC and SEM analysis. The results obtained clearly reveals that metal ions like Cu and Zn induces concentration dependent conformational changes in GR; YMP restores these alterations in way decreasing the effective concentration of metal ions.
谷胱甘肽还原酶（GR）是一种黄素蛋白，可在辅酶 NADPH 的存在下催化氧化型谷胱甘肽（GSSG）还原为还原型谷胱甘肽（2GSH）。谷胱甘肽的重要性源于它在各种代谢过程中起着重要作用。生长在高度污染地区的植物会暴露于更高浓度的金属离子中；从而感受到非生物胁迫，并影响各种调节酶的活性。在这项研究中，研究了金属离子对 GR 的影响。植物半胱氨酸蛋白酶抑制剂在非生物胁迫条件下表达增加。在这里，利用 GR 活性测定、紫外吸收光谱、荧光光谱、FTIR、CD、ITC 和 SEM 分析，研究了从黄芥菜种子中分离得到的半胱氨酸蛋白酶抑制剂对重金属诱导的 GR 构象变化的影响。结果清楚地表明，像 Cu 和 Zn 这样的金属离子会引起 GR 的浓度依赖性构象变化；YMP 通过降低金属离子的有效浓度来恢复这些变化。
