Department of Bio-resources and Food Science, Konkuk University, Seoul, 143-701, South Korea.
Department of Bio-resources and Food Science, Konkuk University, Seoul, 143-701, South Korea.
Food Chem Toxicol. 2019 Mar;125:313-321. doi: 10.1016/j.fct.2019.01.013. Epub 2019 Jan 14.
Dianthus superbus (DS) is a traditional medicinal herb well known for its medicinal and therapeutic potential and widely distributed in various Asian countries. The ethyl acetate (EA), butanol (Bu) and distilled water (DW) extracts of DS assessed for extraction of bioactive compounds and their biological activities. The chemical analysis was done using LC-MS/MS and antioxidant, anticancer and antiviral activities were determined. EA extracts showed strong anticancer activity with IC of 9.5, 13.8 and 69.9 μg/mL on SKOV, NCL-H1299 and Caski cancer cell lines, respectively. The Bu extracts exhibited strongest antiviral activity with respect to both influenza A and B viruses with IC values of 4.97 and 3.9 μg/mL, respectively. Also the metabolic profile for EA, Bu and DW extracts shows high variations and influence precisely the antioxidant, anticancer and antiviral properties. The quercetin 3- rutinoside and isorhamnetin 3- glucoside showed higher neuraminidase inhibition activity in dose dependent manner. Molecular docking study revealed that flavonol glycosides have higher binding activities towards influenza polymerase membrane glycoprotein. Correlation study showed that flavonol glycosides were linked to anti-influenza activity and cyclic peptides with anticancer activities. This study provides vital information for effective utilization of DS for medicinal, food and therapeutic purposes.
香石竹（DS）是一种传统的药用植物，以其药用和治疗潜力而闻名，广泛分布于亚洲各国。评估了 DS 的乙酸乙酯（EA）、正丁醇（Bu）和蒸馏水（DW）提取物以提取生物活性化合物及其生物活性。使用 LC-MS/MS 进行了化学分析，并测定了抗氧化、抗癌和抗病毒活性。EA 提取物对 SKOV、NCL-H1299 和 Caski 癌细胞系的抗癌活性较强，IC 分别为 9.5、13.8 和 69.9μg/mL。Bu 提取物对甲型和乙型流感病毒均表现出最强的抗病毒活性，IC 值分别为 4.97 和 3.9μg/mL。此外，EA、Bu 和 DW 提取物的代谢谱显示出高度的变化，并精确影响抗氧化、抗癌和抗病毒特性。槲皮素 3-芸香糖苷和异鼠李素 3-葡萄糖苷表现出更高的神经氨酸酶抑制活性，呈剂量依赖性。分子对接研究表明，类黄酮糖苷对流感聚合酶膜糖蛋白具有更高的结合活性。相关性研究表明，类黄酮糖苷与抗流感活性有关，环肽与抗癌活性有关。本研究为 DS 在药用、食品和治疗方面的有效利用提供了重要信息。
