Zhang Chenwei, Li Yao, Huo Yue, Wang Hongtao, Wang Dandan
College of Life Sciences, Yantai University, Yantai 264005, China.
School of Biological Science and Food Engineering, Chuzhou University, Chuzhou 239000, China.
Foods. 2025 Jun 30;14(13):2327. doi: 10.3390/foods14132327.
We synthesized QM-AgNPs ( L.-AgNPs, Qu Mai-AgNPs) by an economical and environmentally friendly method using L. extract as a reducing and stabilizing agent. The resulting QM-AgNPs were comprehensively characterized and evaluated for their antioxidant, cytotoxic, and antibacterial activities. Herein, TEM analysis revealed that the QM-AgNPs were predominantly spherical, polydisperse, and exhibited a core particle size ranging from 11 to 18 nm. In contrast, DLS analysis showed a larger hydrodynamic diameter (primarily 60-87 nm), reflecting the hydrated shell and surface biomolecular corona. The crystalline nature of QM-AgNPs was confirmed by XRD and SAED spectra while FTIR spectroscopy indicated the presence of functional groups from the plant extract that may contribute to nanoparticle stabilization. Functional assessments demonstrated that QM-AgNPs exhibited strong antioxidant activity, with efficient DPPH radical scavenging, and selective cytotoxicity against A549 cancer cells while sparing normal cells. Moreover, QM-AgNPs showed significant antibacterial activity against both (Gram-positive) and (Gram-negative), likely due to membrane disruption and the leakage of intracellular contents. To explore practical applications, we developed a GEL@AgNPs coating system for the postharvest preservation of grapes. As a result, the reduced weight loss and decay rate suggest a potential role for QM-AgNPs in extending fruit freshness. Comprehensive shelf-life studies are planned to further substantiate the potential of QM-AgNPs as an effective material for active food packaging applications.
我们使用瞿麦提取物作为还原剂和稳定剂，通过一种经济环保的方法合成了瞿麦-银纳米粒子（L.-AgNPs，瞿麦-银纳米粒子）。对所得的瞿麦-银纳米粒子进行了全面表征，并评估了它们的抗氧化、细胞毒性和抗菌活性。在此，透射电子显微镜（TEM）分析表明，瞿麦-银纳米粒子主要呈球形，多分散，核心粒径范围为11至18纳米。相比之下，动态光散射（DLS）分析显示流体动力学直径更大（主要为60 - 87纳米），这反映了水合壳和表面生物分子冠层。X射线衍射（XRD）和选区电子衍射（SAED）光谱证实了瞿麦-银纳米粒子的晶体性质，而傅里叶变换红外光谱（FTIR）表明植物提取物中存在可能有助于纳米粒子稳定的官能团。功能评估表明，瞿麦-银纳米粒子表现出较强的抗氧化活性，具有高效的二苯基苦味酰基自由基（DPPH）清除能力，对A549癌细胞具有选择性细胞毒性，同时对正常细胞无害。此外，瞿麦-银纳米粒子对金黄色葡萄球菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）均表现出显著的抗菌活性，这可能是由于膜破坏和细胞内物质泄漏所致。为了探索实际应用，我们开发了一种用于葡萄采后保鲜的凝胶@银纳米粒子涂层系统。结果，重量损失和腐烂率的降低表明瞿麦-银纳米粒子在延长水果保鲜期方面具有潜在作用。计划进行全面的货架期研究，以进一步证实瞿麦-银纳米粒子作为活性食品包装应用有效材料的潜力。
