Department Biotechnology and Molecular Biology, University of Opole, ul. Kard. B. Kominka 6a, 45-032 Opole, Poland.
Faculty of Chemistry, University of Opole, Oleska 48, 45-052 Opole, Poland.
Molecules. 2019 Jan 16;24(2):311. doi: 10.3390/molecules24020311.
The aim of the study was to determine the chemical composition of lemon, rosewood, geranium and rosemary oils, and compare their effect on the sensitivity of ZALF 24 and ZALF 339 isolated from infected cereals. The tested oils were added to Potato Dextrose Agar (PDA) medium at concentrations of 0.125%, 0.25%, 0.5%, 1.0% and 2.0%. The activity of the oils on inhibition of the linear growth of mycelium was evaluated by measuring the growth of fungal colonies (growth index), while the fungistatic activity was evaluated on the basis of the percentage growth inhibition of a fungal colony and calculated according to Abbott's formula. The sensitivity of the test strains was variable and depended on the type and concentration of the tested oils. Geranium and rosewood oils in all of the concentrations completely inhibited the growth of the used isolates. In contrast, lemon oil relative to ZALF 339 showed the highest activity at a concentration of 1.0% and rosemary oil, 0.5%. The highest activity against ZALF 24 was shown by the oils of rosemary and lemon at concentrations from 1.0% to 2.0%. The susceptibility of isolates was differentiated and depended on the type and concentration of tested oils.
本研究旨在确定柠檬、紫檀、天竺葵和迷迭香精油的化学成分，并比较它们对从感染谷物中分离的 ZALF 24 和 ZALF 339 的敏感性的影响。将测试油以 0.125%、0.25%、0.5%、1.0%和 2.0%的浓度添加到马铃薯葡萄糖琼脂（PDA）培养基中。通过测量真菌菌落的生长（生长指数）来评估油对菌丝线性生长的抑制活性，而基于真菌菌落生长抑制的百分比来评估抑菌活性，并根据 Abbott 公式进行计算。测试菌株的敏感性是可变的，取决于所测试油的类型和浓度。天竺葵和紫檀油在所有浓度下均完全抑制了所用分离物的生长。相比之下，柠檬油相对于 ZALF 339 在 1.0%的浓度下表现出最高的活性，而迷迭香油在 0.5%的浓度下表现出最高的活性。对 ZALF 24 表现出最高活性的是浓度为 1.0%至 2.0%的迷迭香和柠檬油。分离物的敏感性存在差异，取决于所测试油的类型和浓度。
