Institute of Environmental Engineering and Biotechnology, Faculty of Natural and Technical Sciences, University of Opole, Kominka 6/6A, 45-035 Opole, Poland.
Institute of Biology, Faculty of Natural and Technical Sciences, University of Opole, Oleska 22, 45-052 Opole, Poland.
Molecules. 2021 Oct 27;26(21):6488. doi: 10.3390/molecules26216488.
The aim of the study was to determine the effectiveness of selected seven commercial essential oils (EsO) (grapefruit, lemongrass, tea tree (TTO), thyme, verbena, cajeput, and ) on isolates of common Central European parasitic fungal species of obtained from infected wheat kernels, and to evaluate the oils as potential natural fungicides. The study was conducted in 2 stages. At each stage, the fungicidal activity of EsO (with concentrations of 0.025; 0.05; 0.125; 0.25; 0.50; 1.0, and 2.0%) against spp. was evaluated using the disc plate method and zones of growth inhibition were measured. At the first stage, the fungistatic activity of EsO was evaluated against four species of from the Polish population ( FAPL, FCPL, FGPL and FOPL). The correlation coefficient between the mycelial growth rate index (T) and the fungistatic activity (FA) was calculated. At the second stage, on the basis of the mycelium growth rate index, the effectiveness of the EsO in limiting the development of isolates from the German population ( FC1D, FC2D, FG1D, FG2D and FP0D) was assessed. The first and second stage results presented as a growth rate index were then used to indicate essential oils (as potential natural fungicides) effectively limiting the development of various common Central European parasitic species spp. Finally, the sensitivity of four isolates from the Polish population and five isolates from the German population was compared. The data were compiled in STATISTICA 13.0 (StatSoft, Inc, CA, USA) at the significance level of 0.05. isolates from the German population were generally more sensitive than those from the Polish population. The sensitivity of individual species varied. Their vulnerability, regardless of the isolate origin, in order from the most to the least sensitive, is as follows: , , , and . The strongest fungicidal activity, similar to Funaben T, showed thyme oil (regardless of the concentration). Performance of citral oils (lemongrass and ) was similar but at a concentration above 0.025%.
本研究的目的是确定七种商业精油（EsO）（葡萄柚、柠檬草、茶树（TTO）、百里香、马鞭草、卡宴和）对从中欧常见寄生虫真菌感染的小麦籽粒中分离出的真菌的有效性，并评估这些油作为潜在天然杀菌剂的潜力。研究分两个阶段进行。在每个阶段，使用圆盘法评估 EsO（浓度为 0.025；0.05；0.125；0.25；0.50；1.0 和 2.0%）对 spp. 的杀菌活性，并测量抑菌区的生长抑制情况。在第一阶段，评估了 EsO 对来自波兰种群的四种真菌（FAPL、FCPL、FGPL 和 FOPL）的抑菌活性。计算了菌丝生长率指数（T）和抑菌活性（FA）之间的相关系数。在第二阶段，根据菌丝生长率指数，评估了 EsO 对来自德国种群的 分离株（FC1D、FC2D、FG1D、FG2D 和 FP0D）的发展的有效性。将第一和第二阶段的结果表示为生长率指数，然后用于指示精油（作为潜在的天然杀菌剂）有效限制了各种常见的中欧寄生虫物种 spp. 的发展。最后，比较了来自波兰种群的四个 分离株和来自德国种群的五个 分离株的敏感性。数据使用 STATISTICA 13.0（StatSoft，Inc，CA，USA）在 0.05 的显著水平下进行编译。来自德国种群的 分离株通常比来自波兰种群的分离株更敏感。个别 物种的敏感性不同。无论分离株的来源如何，它们的易感性从最敏感到最不敏感的顺序如下： 、 、 、 和 。百里香精油（无论浓度如何）表现出最强的杀菌活性，类似于 Funaben T。柠檬草和 精油的性能相似，但在浓度高于 0.025%时。
