UR1264 Mycology and Food Safety Research Unit (MycSA), INRAE, F-33882 Villenave d'Ornon, France.
Laboratory Molecules, Material and Applications (LMMA), Preparatory Institute of Scientific and Technical Studies of Tunis (IPEST), University of Carthage, Sidi Bou Said road, La Marsa B.P. 51 2070, Tunisia.
Molecules. 2021 Nov 16;26(22):6906. doi: 10.3390/molecules26226906.
Enniatins are mycotoxins produced by species contaminating cereals and various agricultural commodities. The co-occurrence of these mycotoxins in large quantities with other mycotoxins such as trichothecenes and the possible synergies in toxicity could lead to serious food safety problems. Using the agar dilution method, was selected among eight Tunisian plants for the antifungal potential of its essential oil (EO) on mycelial growth and its production of enniatins. Two EO batches were produced and analyzed by GC/MS-MS. Their activities were measured using both contact assays and fumigant tests (estimated IC50 were 0.1 µL·mL and 7.6 µL·L, respectively). The EOs and their volatiles inhibited the germination of spores and the mycelial growth, showing a fungistatic but not fungicidal activity. The accumulation of enniatins was also significantly reduced (estimated IC50 were 0.05 µL·mL for the contact assays and 4.2 µL·L for the fumigation assays). The most active batch of EO was richer in thymol, the main volatile compound found. Thymol used as fumigant showed a potent fungistatic activity but not a significant antimycotoxigenic activity. Overall, our data demonstrated the bioactivity of EO and its high potential to control and its enniatins production in agricultural commodities.
恩镰菌素是由污染谷物和各种农产品的物种产生的霉菌毒素。这些霉菌毒素与其他霉菌毒素（如单端孢霉烯族化合物）大量共存，并且可能存在协同毒性，这可能导致严重的食品安全问题。使用琼脂稀释法，从 8 种突尼斯植物中选择了一种植物，研究其精油（EO）对其菌丝生长和恩镰菌素产生的抗真菌潜力。生产了两种 EO 批次，并通过 GC/MS-MS 进行了分析。使用接触测定法和熏蒸测定法（估计 IC50 分别为 0.1 µL·mL 和 7.6 µL·L）测量了它们的活性。两种 EO 及其挥发物均抑制了孢子的萌发和菌丝的生长，表现出抑菌但非杀菌活性。恩镰菌素的积累也显著减少（接触测定法的估计 IC50 为 0.05 µL·mL，熏蒸测定法的估计 IC50 为 4.2 µL·L）。最活跃的 EO 批次中含有更多的百里香酚，这是发现的主要挥发性化合物。作为熏蒸剂使用的百里香酚表现出很强的抑菌活性，但对抑制产毒作用没有显著效果。总的来说，我们的数据证明了 的 EO 的生物活性及其在控制农产品中 和其恩镰菌素产生方面的巨大潜力。
